中文摘要：

目的采用毛细管电泳法研究并建立葛根药材的指纹图谱。方法微乳液电动毛细管色谱法。未涂层熔融石英毛细管（63．5cm×50．0cm，50．0μm），缓冲液为微乳液，电压20kv，检测波长254nm。结果以葛根素峰为参照峰，建立了广西葛根药材的微乳液电动毛细管色谱指纹图谱，确认了13个共有特征指纹峰，经加样法标识的成分是大豆苷、葛根素、大豆苷元和染料木素。评价了3个不同产地和4种不同提取方法所得葛根指纹图谱的相似度均在0．97以上。结论本方法具有较好的专属性、精密度和重现性，不仅可增强对极性相似物质的有效分离，用于葛根药材的质量控制，而且其微乳液滴还具有模拟人体生物膜（脂盾体）的作用机制，适用于对体内或体外生物样品的分离分析，具有良好的应用前景。

英文摘要：

OBJECTIVE To research and construct the capillary electrophoresis （CE） characteristic fingerprint of Radix Puerariae. METHODS The separation effects of capillary zone electrophoresis （CZE）, micellar electrokinetic capillary chromatography （MECC or MEKC） and microemulsion electrokinetic capillary chromatography （MEEKC） were examined by CE, respectively. The MEEKC was used as an optimal separation mode. The ultrasonic-assisted extraction, decoction, microwave-assisted extraction and enzyme method were screened and optimised. The ultrasonic-assisted extraction was determined as an optimal extraction method. The total length of capillary column was 63.5 cm, the effective length was 50.0 cm, the inner diameter was 50.0 μm. The detection wavelength was 254 nm： the voltage was 20 kV. Injection time with electromigration was 20 kV x 10 s. RESULTS 10 Batches of Radix Puerariae from Guangxi were selected. The MEEKC characteristic fingerprint of the extract of Radix Puerariae was established. Puerarin peak was used as one reference peak. 13 common characteristic peaks were determined and 4 peak were marked as daidzin, puerarin, daidzein and genistein by external standard method. The similarities of MEEKC characteristic fingerprints from 3 resources of Radix Puerariae and 4 different extraction methods were not less than 0.97. CONCLUSION This method achieved by MEEKC had good specificity, precision and reproducibility. It could be used for the quality control of Radix Puerariae. The microemusion droplets of Radix Pueruriae has special mechanism of action on simulating human body biomembrane （liposome）.