中文摘要：

选择肺癌组织等多种生物组织为代表性样品,在正离子检测模式下,以磷脂类物质丰度、信号强度高为目标,考察了21种不同组成的CH3OH/H2O溶剂体系,获得了最佳溶剂（CH3OH-H2O,30∶70,V/V）,建立了一种无需破坏、研磨即可获取组织内部样品中磷脂类物质信号的内部萃取电喷雾电离质谱（i EESI-MS）方法,并成功应用于肺癌、食管癌、猪肉、牛肉、猪肺、猪心等不同生物组织样品中磷脂类物质的直接质谱分析。本方法无需样品预处理,可通过选择合适的萃取溶剂来提高方法的分析灵敏度和选择性,单个样品的平均分析时间少于1 min,样品耗量少,有望为生物组织样品中磷脂类物质的研究提供一种质谱学新方法。

英文摘要：

Phospholipids and their metabolites play an important role in a variety of cellular processes including cell-cell adhesion,cell growth and differentiation,apoptosis,phagocytosis as well as storage of energy. In this study,the phospholipid composition of cancer tissue and adjacent normal tissue from humans and animals were analyzed by internal extractive electrospray ionization mass spectrometry（ i EESI-MS）.Extractive solvent at high voltage（ ＋5. 5 k V） was injected into tissue samples using a fused silica capillary at a flow rate of 0. 5-1 μL / min,producing fine charged droplets containing analytes of tissue samples at the tip of the sample. Charged droplets were directly sampled to the atmospheric inlet of a mass spectrometer. Out of 21 different ratios of CH3 OH ∶ H2 O solvent mixture,the ratio CH3 OH ∶ H2 O = 30 ∶ 70（ V / V） showed the optimal phospholipids extraction and visibility in MS. A large number of phospholipids from different tissue samples（ such as cancer tissue and adjacent normal tissue of lung cancer,esophageal cancer tissue,pork,beef,porcine heart and porcine lung） were obtained simultaneously by i EESI-MS analysis. The experimental results demonstrated that i EESI-MS was characterized by minimal sample pretreatment, low sample consumption,and rapid analysis（ the analysis time per sample was less than 1 min）,and the selectivity and sensitivity of i EESI-MS could be improved by choosing proper solvent. Importantly,the experimental results provided new information for further studies of phospholipids in biological tissues.