中文摘要：

生物体内半胱氨酸（L-Cys）浓度异常会导致多种疾病的发生,因此半胱氨酸的高灵敏检测具有重要的临床意义。分别将市售二硫化钼粉末和过氧化氢作为前体和氧化剂成功地制备了具有强荧光的氧化钼量子点（MoOX·QDs）,并发现铜离子可猝灭氧化钼量子点的荧光。而后,根据巯基与铜离子间的特异相互作用,加入L-Cys后荧光得到恢复。以氧化钼量子点作为新型荧光探针,建立了高灵敏和高选择性的半胱氨酸检测方法。在优化后的实验条件下,当半胱氨酸浓度为5-25μmol·L^-1时,体系的荧光恢复程度与半胱氨酸浓度间呈现良好的线性关系,线性相关系数R^2=0.99。可重复性好,且其他氨基酸干扰小,可应用于水样或混合氨基酸合成样品中半胱氨酸的检测,为以后临床医学人体疾病的诊断打开了新思路。

英文摘要：

The abnormal concentration of cysteine （L-Cys） in the body can lead to a variety of diseasing,so the high sensitive detection of cysteine was significance important for clinical.Molybdenum oxide quantum dots were prepared using commercial molybdenum disulfide powder and hydrogen peroxide as the precursor and oxidant respectively.The addition of Cu2＋ will quench the fluorescence of molybdenum oxide quantum dots.Then,a new method with high sensitivity and selectivity was developed for cysteine determination based on molybdenum oxide quantum dots,which was according to the specific interaction between thiol and copper ions.Under the optimized conditions,there was a good linear relationship between the fluorescence intensity and the content of L-Cys ranging from 5 μmol·L-1 to 25 μmol·L^-1 with a correlation coefficient of R^2=0.99.This method was simple,exactitude,reproducible and can be used for the detection of cysteine in water or mixed amino acid synthetic samples.It can be used as the basis of certain diseases in the medical diagnosis.