中文摘要：

本研究以721矿和745矿嗜酸性氧化亚铁硫杆菌为研究对象,采用常压化学电离质谱直接分析其代谢产物,分别考察了顶空采样（Headspace sampling）、界面采样（Interface sampling）和中性解吸采样（Neutral desorption sampling）3种进样方式对电离效果的影响。在优化条件下,常压化学电离质谱对微生物纯菌种和混合菌种的代谢产物均具有良好的分析能力,可根据获得的代谢产物指纹谱图结合主成分分析（PCA）方法和聚类分析（CA）方法区分2个放射性强弱不同区域共4类嗜酸性微生物样品,并对主要胺类、酯类等代谢成分进行串联质谱鉴定,为耐辐射微生物的相关研究提供了一种可借鉴的分析方法。

英文摘要：

A method for rapid determination of acidithiobacillus ferrooxidans（At. f）metabolites by ambient corona discharge ionization mass spectrometry was established. Three injection modes were applied to study the effects such as headspace sampling,interface sampling and neutral desorption sampling. Under the optimized experimental conditions,the metabolites,such as small molecule esters and amines were detected by ambient corona discharge ionization mass spectrometry and confirmed using tandem mass spectrometry（MS / MS）.Further principal component analysis（PCA）and cluster analysis（CA）of the mass spectrometric results allowed a confident discrimination of different bacterial samples. Ambient corona discharge ionization mass spectrometry has the advantages of no sample pretreatment,convenient operation,high sensitivity and high analytical speed,which will be an attractive method to rapidly identification of microorganisms.