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中文摘要：
通过向体外-培养的新生犊牛肝细胞添加非酯化脂肪酸（NEFA）、β-羟丁酸（BHBA）和葡萄糖，采用内对照RTPCR方法检测代谢产物对体外培养新生犊牛肝细胞MTPmRNA丰度的影响。结果显示，随着培养液中NEFA和葡萄糖浓度升高，MTPmRNA的丰度呈现剂量依赖性上调（P〈0．01）；随着BHBA浓度的增加，MTPmRNA的丰度先升高后降低（P〈0．01或P〈0．05）。表明，NEFA和葡萄糖对肝细胞MTPmRNA表达具有促进作用，呈剂量依赖性；BHBA对肝细胞MTPmRNA表达具有低剂量促进高剂量抑制的双重作用。
英文摘要：
Different concentrations of nonesterified fatty acids（NEFA）,β-hydroxybutyric acid（BHBA） and glucose were added into the culture media with monolayer of primary-cultured newborn calf hepatocytes respectively. After 36 hours, abundance of MTPmRNA in primary-cultured newborn calf hepatocytes was detected by semiquantitative RT-PCR. The result showed that with increasing concentrations of NEFA and glucose,abundance of MTPmRNA in hepatocytes was up-regulated significantly with dose-dependence （P〈0.01）. However, with increasing concentrations of BHBA, abundance of MTPmRNA in hepatocytes was up-regulated, and then decreased（P〈0.01 or P〈0. 05）. It was concluded that metabolic products such as NEFA,BHBA and glucose could regulate expression of MTPmRNA.
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