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中文摘要：
利用新生犊牛大网膜的脂肪组织原代培养脂肪细胞,待细胞贴满培养平皿底部90%时,进行细胞传代。观察细胞的形态;绘制细胞生长曲线、脂肪含量变化曲线;对细胞进行冻存与复苏,并计算复苏后的存活率。结果显示,细胞传代培养后,仍保持脂肪细胞蓄积脂肪的特性,细胞生长曲线与原代脂肪细胞的生长曲线依然一致,细胞冻存复苏后保持87%的存活率。
英文摘要：
To understand calf preadipocytes activities by serial subcultivation,the primary culture preadipocytes from calf epiploica were proliferated and passaged when the cell growth and attachment arrived 90%.During serial subcultivation,it was done to observe cellular dynamic morphological changes,draw growth curve by lencocyte count,survey fat content by extracting oil red O from the stained cells.The cellular survival rate after being freezed was counted by trypen-blue-stain.The results are as follow:the cells ...
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