中文摘要：

利用300个EST-SSRs标记对普通二倍体鲫（Carassius auratus）自交F2代的181个个体进行基因型检测,并对其体重、体厚两种经济性状进行单标记回归分析。Permutation检验（10 000次）结果显示,38个标记与所检测的经济性状相关,其中26个标记达到显著性相关（P〈0.05）,12个标记达到极显著性相关（P〈0.01）。在38个相关标记中,有27个标记与体重相关,25个标记与体厚相关,14个标记与体重、体厚均相关。对同一标记的不同基因型之间进行多重比较,找到了与两种经济性状相关的基因型。将与性状相关的38个标记与斑马鱼（Da-nio rerio）基因组序列进行BLAST同源性比对,找到10条相似性高且功能已知的EST-SSRs序列（E〈e-20）。其中,JE594与斑马鱼膜结合环指蛋白基因march7相似性高达95%,JE3165与斑马鱼肌盲蛋白基因mbnl1的相似性达78%,JE6996与斑马鱼促蛋白激酶的基因mapk10的相似性高达98%,JE4984与斑马鱼蛋白磷酸酶的基因ppp1cab相似性达84%。本研究筛选出了与鲫体重、体厚相关的EST-SSRs标记及基因型并找到了与鲫生长相关的候选功能基因,为鲫的分子标记辅助育种（MAS）及目标性状的遗传改良提供依据。

英文摘要：

300 EST-SSRs markers were selected to analyze the genomic DNA of 181 progenies from a normal Carassius au- ratus F2 family, and two growth traits （body weight and body width） were conducted single marker regression analysis. 38 EST-SSRs markers were found to be closely related to growth traits, 26 of them were significantly associated with growth traits （P 〈 0. 05 ）, the other 12 markers had a highly significant correlation with growth traits （P 〈 0. 01 ） ; In these 38 markers, 27 markers were related with body weight, 25 markers were related with body width, and 14 markers were relat- ed with both body weight and width. Meanwhile, the genotypes of these correlative loci were determined. Furthermore,by using BLAST online in NCBI, the homology identity of these 38 EST-SSRs markers was determined. Results showed that, 10 markers had significant similarity with the sequences in GenBank （E 〈 e-20 ）. JE594 was highly homologous （95%） to the gene encoding membrane-associated ring finger protein of Danio rerio; JE3165 was highly homologous （78%） to the gene encoding muscleblind-like protein of D. rerio; JE6996 was highly correlated （98%） with the gene en- coding mitogen-activated protein kinase of D. rerio; JE4984 was highly correlated （84%） with the gene encoding protein phosphatase 1 of D. rerio. These loci may be used for marker-assisted selection and genetic improvement.