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一个镜鲤家系的遗传多样性及经济性状的遗传连锁分析
  • ISSN号:1005-3832
  • 期刊名称:《水产学杂志》
  • 时间:0
  • 分类:Q953[生物学—动物学]
  • 作者机构:[1]中国水产科学研究院黑龙江水产研究所,农业部水产生物技术重点开放实验室,黑龙江哈尔滨150070, [2]澳大利亚国立大学,堪培拉
  • 相关基金:国家重点基础研究发展计划(973计划)2010CB126305; 公益性行业(农业)科研专项(200903045)
中文摘要:

本试验在一个镜鲤家系中,随机选取68尾作为实验鱼,在测量体重、体长、全长等数量性状的同时,利用15个微卫星分子标记对其进行遗传检测,共检测到40个等位基因,每个基因座的等位基因数为1~4个不等,平均等位基因2.66个,片段长度在132-551之间,有效等位基因数(Ne)为1.3721~3.8278,平均观察杂合度(Ho)为0.3235~1.0000,平均期望杂合度(He)为0.3464~0.7442,平均多态信息含量(PIC)为0.4754。结果表明:该镜鲤家系的遗传多样性处于中等水平,但在较大的选择压力下已严重偏离Hardy-Wenberg平衡。利用SPSS程序下的GLM过程对15个微卫星位点与主要经济性状的相关性进行分析,结果发现:HLJ021、HLJ354、HLJ551共3个微卫星位点对镜鲤体重显著影响(p〈0.05),其中,位点HLJ021、HLJ044、HLJ551、HLJ1330还对体高存在显著影响(p〈0.05),HLJ354对体长、体厚、全长存在显著影响(p〈0.05)。本研究所鉴定的与生长性状相关的标记可为德国镜鲤分子标记辅助育种提供重要信息。

英文摘要:

Fifteen microsatellite markers were used to analyze five economic traits including body weight,body length,total length,body width and body height in 68 samples from a mirror carp(Cyprinus carpio) family.A total of 40 different alleles were detected and each locus ranged from 1 to 4i n number of alleles with average allele number of 2.67.The DNA fragment lengths were found to vary from 132 bp to 551 bp.In the one family,mean valid alleles(Ne) was found to range from 1.3721-3.8278 in number,the value of average observed(Ho) from 0.3235 to 1.0000 and expected heterozygosity(He) from 0.3464 to 0.7442.The mean value of polymorphic information content(PIC) of these microsatellite loci was found to be 0.4754,indicating that this family had a moderate level of genetic variability. A GLM procedure in the SPSS software was used to analyze the effects of these 15 microsatellites markers on economic characters.A total of five markers were found to be closely related to these traits,in which 3 markers,HL021,HLJ354 and HLJE551,had a significant impact on body weight(p0.05).The HLJ021、HLJ044、HLJ551 and HLJ1330 loci were found to be significantly correlated with length character(p0.05).The HLJ354 loci was found to be significantly correlated with body length,body width,and total length character(p0.05).The microsatellite markers used in our study might be used as a marker assisting selection for growth trait.

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期刊信息
  • 《水产学杂志》
  • 主管单位:中国水产科学研究院
  • 主办单位:中国水产科学研究院
  • 主编:石连玉
  • 地址:哈尔滨市道里区河松街232号
  • 邮编:150070
  • 邮箱:zazhi2000@126.com
  • 电话:0451-84861626
  • 国际标准刊号:ISSN:1005-3832
  • 国内统一刊号:ISSN:23-1363/S
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录
  • 被引量:3021