中文摘要：

以亚麻茎部组织总RNA为模版,通过反转录PCR获得糖基转移酶Lu UGT72E1基因的全长开放读码框序列。序列分析结果表明：开放读码框全长1 476bp,共编码491个氨基酸。通过多氨基酸比对发现,该蛋白与亚麻UGT72N2蛋白亲缘关系最近,相似度达99%,与毛果杨、巨桉、木薯、雷蒙德氏棉及拟南芥UGT72E1蛋白的氨基酸序列相似性在58%-51%。分子进化分析表明,该蛋白与拟南芥UGT72E1-3聚为一类,推测该蛋白参与木质素单体的糖基化修饰过程。基因表达谱结果表明,该基因被BR、Brz诱导下调表达。本研究为亚麻的纤维品质改良提供了候选基因。

英文摘要：

The total RNA extracted from flax stems was used for RT-PCR, then the full-length open reading frame（ORF） sequence of glycosyltransferase gene（Lu UGT72E1） was amplified. The sequence analysis showed that the fulllength ORF of Lu UGT72E1 consisted of 1 476 bp, encoding 491 amino acids. Homology analysis of the deduced amino acid showed 99% identity to UGT72N2 from flax and 58% to 51% identity to UGT72E1 from Populus trichocarpa, Eucalyptus grandis, Manihot esculenta, Gossypium raimondii and Arabidopsis thaliana. The phylogeny analysis showed that this protein clustered with UGT72E1-3 from Arabidopsis and might play key roles in the process of monolignols glucosylation. The gene expression profiling showed that Lu UGT72E1 gene could be induced by BR and Brz. This study could provide candidate genes for fibre improvementin flax.