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Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays
  • ISSN号：1672-7681
  • 期刊名称：《中国免疫学杂志：英文版》
  • 时间：0
  • 分类：R392[医药卫生—免疫学;医药卫生—基础医学]
  • 作者机构：[1]The State Key Laboratory of Pharmaceutical Bioteehnology and JiangsuCenter of Hepatobiliary Diseases, Nanjing University, Nanjing 210093,China
  • 相关基金：The project was financially supported by the following funds to Zi-Chun Hua： the National Nature Science Foundation of China （No. 30425009, 30600320, 30730030, 30330530, 30270291）, Jiangsu Provincial Nature Sciences Foundation （No. BK2007715） and Jiangsu Provincial Department of Health （No. H200524, H200742）.
作者： Faiz MMT Marikar Dingyuan Ma Jianqiang Ye Bo Tang Weijuan Zheng Jing Zhang Min Lu Zichun Hua[1]
关键词： FADD, 多细胞抗体, 免疫荧光测定, 基因表达, FADD, His-tag fusion protein, polyclonal antibody, immunofluorescence assay
中文摘要：

野类型的人的联系船边交货的死亡领域(FADD ) 蛋白质在 Escherichia coli 被表示为他的标签熔化蛋白质。Recombinant FADD 蛋白质在使中毒的条件下面被净化。在使中毒的蛋白质纯化以后，它是 refolded 并且在大约 23 mg/L 的收益获得了。净化了作为相应于 31 kDa 的分子的重量的一个同质的乐队展出的 FADD。对 refolded FADD 蛋白质的兔子的免疫被给予高 titre polyclonal 抗血清的生产。这新 polyclonal 抗体能在西方的污点认出 recombinant FADD 蛋白质。Immunoreactivity 也在 immunofluorescence 试金被观察。低费用 polyclonal 抗血清对在各种各样的免疫分析的 FADD 的广泛的察觉适用。

英文摘要：

The wild-type human Fas-associated death domain （FADD） protein was expressed as a His-tag fusion protein in Escherichia coll. Recombinant FADD proteins were purified under the denatured condition. After denatured protein purification, it was refolded and obtained at a yield of about 23 mg/L. Purified FADD exhibited as a homogenous band corresponding to the molecular weight of 31 kDa. Immunization of rabbits against the refolded FADD protein was allowed the production of high titre polyclonal antiserum. This new polyelonal antibody could recognize recombinant FADD protein in Western blot. Immunoreactivity was also observed in immunofiuorescence assay. The low cost polyclonal antiserum was applicable to extensive detection of FADD in various immunoassays. Cellular ＆ Molecular Immunology. 2008;5（6）：471-474.

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期刊信息
  • 《中国免疫学杂志：英文版》
  • 主管单位:
  • 主办单位:中国免疫学会 中国科技大学
  • 主编：
  • 地址：合肥黄山路443号中国科技大学生命科学院
  • 邮编：230027
  • 邮箱：cmi@ustc.edu.cn
  • 电话：0551-3607377
  • 国际标准刊号：ISSN：1672-7681
  • 国内统一刊号：ISSN：11-4987/R
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,荷兰文摘与引文数据库,荷兰医学文摘,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引（扩展库）,美国生物科学数据库
  • 被引量:132