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中文摘要：
为研究山茶重瓣性状形成分子机理,采用RACE技术从完全重瓣型山茶花品种‘红十八学士’中克隆TM6基因全长cDNA,再利用生物信息学和Real-time PCR技术对其进行分析。结果表明：该基因cDNA全长729 bp,命名为CjHTM6,有一个完整的开放阅读框（627 bp）,编码208个氨基酸,蛋白质分子量24.31 ku,理论等电点为9.53,不稳定系数达44.07;编码蛋白为MADS-box蛋白,其预测的二级结构和三级结构主要以α-螺旋为主,其次为无规卷曲,延伸链所占比重最小;与单瓣花型短柄山茶至少有4个氨基酸差异。定量分析结果表明,CjHTM6基因在花中各部均有表达,其中在花心的花瓣中表达量最高,而在苞片中表达量最低。初步表明,CjHTM6基因在山茶重瓣花形成中起重要作用。
英文摘要：
To study the molecular mechanism of double flowers,TM6（TOMATO MADS BOX GENE6）gene cDNA named as CjHTM6 was cloned from Camellia japonica＇Hongshibaxueshi＇,and the full length was amplified by RACE（Rapid amplification of cDNA end）method and analyzed by bioinformatics.These results showed that CjHTM6 cDNA sequence length was 729 bp and it included a completed open reading frame（ORF）encoding 208 amino acids.The molecular weight of the protein by CjHTM6 gene was 2.34 kD,and the iso-electric point was 9.53,and the instability index was 44.07.The coding protein belonged to the MADS-box with mainly alpha-helix,followed by random coil and few extend strand in its tertiary or secondary structure.The amino acids sequence had four differences with Camellia japonica subs.rusticana.The expression results showed that it expressed at every flower parts,but the highest was at central petals,and the lowest was at bracts.The CjHTM6 gene may be involved in formal double thigmomorphogenesis in Camellia.
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