中文摘要：

在疫苗生产中需要对培养病毒的有效抗原数量进行定量，对其一般采用TCID50测定或噬斑滴定的方法。这些传统方法的缺点是耗时、重复性差异较大。本研究采用qRT-PCR检测蓝舌病病毒含量，并用准确滴定噬斑单位的蓝舌病病毒作为对照，比较待检样品和对照的Ct值，通过线性方程计算待检样品的病毒含量。对7个独立样品的检测结果表明，该方法的准确性和重复性均高于噬斑分析方法，并且可在蓝舌病病毒培养过程中实时检测病毒含量。该方法操作简单、快捷，可在病毒生产过程即时检测病毒含量，在蓝舌病疫苗生产中具有重要意义。

英文摘要：

Quantitatively amount of cultivated virus in vaccine production was necessary,and the method of TCID50or plaques titration to quantitative was adopted generally.The shortcomings of these traditional methods were timeconsumingand the repeatability was not strong.The Bluetongue virus content was detected by qRT-PCR,in contrastwith accurate titration plaques units of Bluetongue virus as a control in this study.The Ct values of test and controlsamples were compared,and the concentrations of test samples by the linear equation was estimated.The test results of7independent samples showed that the accuracy and repeatability were higher than plaques analysis method.And thismethod could be applied to real-time detection virus content in the process of virus culture.The method was simple andrapid during the operation,and could detecte virus content in the process of virus culture,which had significance inBluetongue vaccine production.