中文摘要：

为建立蓝舌病病毒（BTV）群特异性抗体的检测方法，本研究通过制备BTV群特异性多克隆抗体，建立蓝舌病群特异性阻断ELISA抗体检测方法。利用该方法同时对88份绵羊和山羊BTV阴性血清进行检测，确定阴阳性血清临界值为抗原对照平均0D450nm的50％。实验结果表明，该方法的敏感性、特异性和重复性符合通用标准。该方法根据血清样品对特定稀释度病毒抗原的阻断能力确定待检样品的抗体效价，通过阻断ELISA与中和试验对29份标准阳性血清检测结果比较表明：成对样本相关系数为0．898，相关性显著，蓝舌病群特异性阻断ELISA可以取代中和试验用于蓝舌病的血清抗体效价检测。

英文摘要：

In order to establish a group specificity method for detection of the antibody titer against bluetongue virus （BTV）, the group specificity blocking ELISA was developed by polyclonal antibody against BTV. In addition, the cut-off value was set by calibration of 88 serum samples of negative sera flom sheep and goats. This detection assay was proved to be sensitivity, specificity and repeatability. Furthermore, 29 serum samples were detected by both virus neutralization test （VNT） and the established blocking-ELISA, the results showed that the antibody titers detected by the blocking-ELISA titer and the VNT were the similar and no significant different, with the correlation value of 0.898. Therefore, the established blocking-ELISA had the potentially to substitute VNT in BTV antibody detection.