中文摘要：

为了解牛流行热在天祝白牦牛中的流行状况,本研究从甘肃省武威市天祝藏族自治县的7个乡镇分别随机抽样采集了224头白牦牛的抗凝血和血清,应用间接ELISA抗体检测试剂盒检测血清中牛流行热病毒特异抗体,应用实时荧光定量RT-PCR、RT-PCR方法检测牛流行热病毒G基因并进行序列验证。结果表明,7个乡镇的白牦牛均存在牛流行热病毒感染,抗体阳性率为45.09%;荧光定量RT-PCR的检出阳性率为37.5%;以RT-PCR扩增G基因3’端420 bp区段测序并构建系统发育树表明,白牦牛群流行的BEFV毒株与中国大陆1976年分离的JB76H毒株亲缘关系较近,为BEFV基因Ⅰ型。本研究首次证实白牦牛在自然状态下可感染牛流行热病毒,可能是重要的牛流行热病毒宿主。

英文摘要：

In order to investigate the status of bovine ephemeral fever in Tianzhu white yaks,in this study the blood samples were collected from seven towns and tested for BEFV antibodies using indirect ELISA.Total RNA was extracted and viral nucleic acids were detected by real-time RT-PCR,and the G gene was amplified by RT-PCR and further sequenced.In result,45.09% white yaks were positive for BEFV antibodies,and a total prevalence of BEFV RNA was detected in 37.5% of the samples by real-time RT-PCR.A 420 bp fragment of G gene 3＇terminal was amplified and sequenced for phylogenetic analysis,which showed that the BEFV infected in white yaks was closely related to JB76H that was separated from China mainland in 1976 and belonged to subtype I.This study result for the first time provided evidence for natural BEFV infection in white yaks,implying that the white yaks may be an important host of BEFV.