中文摘要：

采用偏最小二乘判别分析法（PLS‐DA）分析不同培养时间中国被毛孢代谢产物的变化。结果表明,在不同培养时间内,供试菌株菌丝的次级代谢产物差异显著。根据代谢组差异可将中国被毛孢培养时间分为前期（19d前）、中期（19–26d）、后期（26d后）3个阶段。3个培养阶段共出现43种显著差异化合物,其中仅在培养前期出现的差异化合物有3种,大部分差异代谢物出现在前期后段及中期,包括8种抗生素及多种具有独特生物活性的物质,在培养后期有3种抗生素没有检测到。在整个培养过程中,有些代谢物可能是受反馈调节机制控制,其含量呈波动变化; 有些是持续合成的稳定的代谢终产物,其含量持续增加; 有些由于合成速度逐渐减慢,或分解速度逐渐加快而出现含量持续下降。从代谢物的利用角度来说,人工培养菌丝适宜在培养中期采收,因为此阶段生物活性代谢物最多,但对于含量持续下降的化合物的利用应在培养前期采收; 对于含量持续增加的化合物的利用宜在培养后期采收。

英文摘要：

The method of partial least squares projection to latent structure-discriminant analysis （PLS-DA） was developed for determination of metabolite difference of mycelia of Hirsutella sinensis incubated on a solid medium in different duration. The results showed that the secondary metabolites of mycelia was significantly different in different ncubation duration. According to the metabolite differences, the incubation process of Hirsutella sinensis was dividedinto three phases： early period （before day 19）, medium period （day 19-26）, and late period （after day 26）. In total, forty three secondary metabolites were detected throughout the incubation periods. Three of them were produced only in the early incubation period. Most of the secondary metabolites appeared in the late time of early period and medium period, including eight antibiotics and several other active substances. Three antibiotics were not detected in the late period. During cultivation, some metabolites with fluctuated concentrations may be controlled by the feedback regulation mechanism. Metabolites with increased concentrations may be stable compounds synthesized continuously by the fungus. Metabolites with decreased concentrations may be due to the slow-down of the synthetic speed or the gradual acceleration of the decomposition speed. In terms of metabolite content, artificially cultivated mycelia of Hirsutella sinensis should be harvested in the medium period due to that most of the active substances were produced in this periods. For the harvest of the compounds with continuously decreased concentrations, the cultured mycelia should be gathered at the early period. For the harvest of the compounds with continuously increased concentrations, the cultured mycelia should be gathered at the late period.