中文摘要：

利用LC-MS对不同培养时间蝉棒束孢菌丝体代谢物进行检测,结合多变量统计分析的主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）鉴定差异化合物。PCA和OPLS-DA表明蝉棒束孢菌丝体中的代谢物随培养时间不同有显著变化。阴、阳离子模式下我们都可以把蝉棒束孢代谢物变化分为前期、中期和后期3个时期。培养前期产生较多的细胞松弛素E、肌醇、γ-氨基丁酸、乙酰肉碱、亚油酸等与侵染宿主、早期信号传导和氧化供能有关的物质;中期产生较多的甘露醇、海藻糖、亚麻酸、柠檬酸、丙酮酸、7,8-二羟基油酸等与抗逆和氧化供能有关的物质,此外还有两种抗生素红酵母红素、δ-氨基-γ-酮戊酸达到了最大值;培养后期产生较多的脯氨酸、2,4-二氨基丁酸、3′-deoxy-hanasanagin、ulvaline、赤霉素、烟酰胺等与抗逆、抗氧化、产孢、细胞保护有关的物质。首次发现烟酰胺可能与蝉棒束孢从营养生长转化为生殖生长及产孢有关;同时赤霉素、ulvaline和δ-氨基-γ-酮戊酸可能与蝉棒束孢产孢或孢子成熟有关,尤其值得注意的是蝉棒束孢在培养后期高速合成和大量积累赤霉素,值得深入研究。根据蝉棒束孢的代谢特点,作为药品及保健品开发蝉棒束孢菌丝产品时,培养时间宜在7–11d。

英文摘要：

Metabolites in the mycelia of Isaria cicadae at different incubation time were determined by LC-MS, and the metabolite differences were identified with multivariate analysis methods such as principal components analysis （PCA） and orthogonal projection on latent structure-discriminant analysis （OPLS-DA）. PCA and OPLS-DA demonstrated that the metabolites n mycelia of Isaria cicadae varied significantly at different incubation time. According to the metabolites in the mycelia of Isaria cicadae analyzed both in positive mode and in negative mode, the incubation t prophase, metaphase and anaphase. In the prophase, Isaria cicadae produced high acetylcarnitine, linoleic acid and so on, which possess the functions of infecting the me can be divided into three periods, i.e er content of cytochalasin E, inositol, GABA, host, early signal transducting and for energy. In the metaphase, the mycelia of Isaria cicadae had higher contents of mannitol, trehalose, linolenic acid, c oxidating itric acid, pyruvic acid, 7,8-dihydroxy-oleic acid and so on, which have the functions of stress tolerance and antioxidant activities. There were also two antibiotics, torularhodin and 5-aminolevulinic acid, which reached the maximum at this period. In the anaphase, the mycelia of Isaria cicadae contained higher contents of proline, 2,4-diaminobutyric acid, 3＇-deoxy-hanasanagin, ulvaline, gibberellin, niacinamide and so on, which have the functions of stress tolerence, antioxidant activity, cytoprotectivity, and sporogenesis. We found that niacinamide were possibly related to the change from vegetative growth to reproductive growth and sporogenesis in Isaria cicadae. The metabolites of gibberellin, ulvaline and 6-aminolevulinic acid were possibly related to sporogenesis or spore maturation. It is worthy of further study of the phenomenon that gibberellin was produced and accumulated very swiftly at the anaphase. According to metabolic characteristics of P. cicadae, it is recommended that the fungus could be incubated for 7-11 days fo