采用同时蒸馏萃取-气相色谱质谱联用(SDE-GC-MS)的方法分析了蝉拟青霉、拟细羽束梗孢、根足被毛孢菌丝的挥发性成分,从中分别鉴定出44、28和19种化合物,它们主要为萜类化合物、芳香族化合物、醇类、烷烃类、酯类和醛类。成分比较发现,3种虫生真菌挥发性物质中有3种主要共有成分,分别为丁羟基甲苯、1-辛烯-3-醇、苯乙醛。除共有成分外,它们各自都有大量特有成分,其中蝉拟青霉主要有5-甲基-2-呋喃-乙酸酯、反式-2,4-癸二烯醛、长叶烯等;拟细羽束梗孢主要有5-羟基-2-癸烯酸-δ-内酯、2,4-二甲基-恶唑、苯酚、β-榄香烯等;根足被毛孢主要有3,4,5-三甲基-苯甲醛、1,3-二甲基-3,4,5,6-四氢化-2(1H)嘧啶、顺式-2-羟基-1-(羟甲基)-9-十八碳一烯酸乙酯。
Volatile components of Paecilomyces cicadae,Isaria gracilioides and Hirsutella heteropoda were extracted by method of simultaneous distillation extraction (SDE),and analyzed with gas chromatography-mass spectrometry (GC-MS).There were 44,28 and 19 compounds were identified from the three species respectively.The main components were terpenoids,aromatic compounds,alcohols,alkanes,esters and aldehydes.Components' comparison revealed that butylated hydroxytoluene,1-octen-3-ol,benzeneacetaldehyde and decamethyl-cyclopentasiloxane existed commonly in all the 3 species.Besides the 3 main common components Paecilomyces cicadae contained uniquely 5-methylfurfuryl acetate,(E,E)-2,4-decadienal and decahydro-4,8,8-trimethyl-9-methylene-1,4-methanoazulene;Isaria gracilioides contained uniquely 5,6-dihydro-6-pentyl-2H-pyran-2-one,2,4-dimethyl-oxazole,phenol and 1-ethenyl-1-methyl-2,4-bis(1-methylethenyl)-cyclohexane,and Hirsutella heteropoda contained uniquely 3,4,5-trimethoxy-Silane,(4-methoxyphenoxy)trimethyl-benzaldehyde,1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone,and 9-octadecenoic acid(Z)-2-hydroxy-1-(hydroxymethyl)ethyl ester.