中文摘要：

建立了用高速逆流色谱从蝙蝠蛾拟青霉中高效、快速分离制备高纯度麦角甾醇的方法。将蝙蝠蛾拟青霉的乙酸乙酯提取物直接进行高速逆流色谱分离,考察了不同溶剂系统的分离效果。结果表明,最佳的溶剂系统为正己烷-乙酸乙酯-甲醇-水（体积比为6∶1.7∶6∶0.3）,以上相为固定相,下相为流动相,转速为850r/min,流速为2mL/min,检测波长为280nm。制备所得的麦角甾醇经紫外光谱（UV）和高分辨质谱（HRMS）鉴定及与标准品对照定性 纯度经高效液相色谱（HPLC）分析为99.2%（峰面积归一化法）。该方法制备麦角甾醇简便、快速,所得产物的纯度高,适合于麦角甾醇对照品的制备。

英文摘要：

To develop an effective and rapid method for the preparation of ergosterol from a strain of Paecilomyces hepialid, the ethyl acetate extract of the mycelia was injected into a high-speed counter-current chromatograph （HSCCC） directly, and eluted with different solvent systems. The result showed that the solvent system composed of n-hexane-ethyl acetate-methanol-water （6∶1.7∶6∶0.3, v/v/v/v） was the best. The lower phase was used as the mobile phase and performed at a flow rate of 2 mL/min, while the apparatus rotated at 850 r/min, and detected at 280 nm. The prepared ergosterol was identified with ultraviolet detection （UV）, high resolution mass spectrometer （HRMS） and standard, and its purity was 99.2% analyzed by high performance liquid chromatography. The established method is relatively simple, fast, and suitable for the large-scale isolation and separation of ergosterol.