中文摘要：

【目的】分析蛹拟青霉（Paecilomyces militaris）菌株RCEF0927发酵液中的抗中国仓鼠卵巢瘤（CHO）细胞活性及活性成分的具体组成。【方法】用刃天青（Resazurin）法测定样品对CHO细胞的抑制率;用高效液相色谱-高分辨质谱和活性测定联用的方法进行活性成分分析和鉴定。【结果】活性测定结果表明菌株RCEF0927的发酵液具有较强的抗CHO细胞活性;提取实验结果表明抗肿瘤活性物质能较好地被乙酸乙酯提取出来;液相色谱-质谱-活性测定分析表明提取物中活性组分的可能分子式分别为C10H13N5O3、C22H22O9、C23H24O10、C22H22O10、C15H10O4、C15H10O5、C36H70O11、C36H68O12、C38H74O11、C40H76O13和C44H82O14。【结论】根据细胞毒性、色谱特性、紫外光谱特征和质谱碎片,可初步推断发酵物中抗肿瘤活性成分分别为虫草素、4′-羟基异黄酮-7-O-（4″-O-甲基）-β-D-葡萄糖甙、6-甲氧基-4′-羟基异黄酮-7-O-（4″-O-甲基）-β-D-葡萄糖甙、5,4′,-二羟基异黄酮-7-O-（4″-O-甲基）β--D-葡萄糖甙、7，4′，-二羟基异黄酮、5，7，4′，-三羟基异黄酮、油酸酯聚氧乙烯醚、聚氧乙烯山梨醇酐单硬脂酸酯脱水化合物、聚氧乙烯山梨醇酐单油酸酯，以及含聚氧丙烯或聚氧丁烯结构单元的聚氧乙烯山梨醇酐脂肪酸酯衍生物等化合物。上述化合物对CHO细胞的毒性为首次发现。

英文摘要：

[Objective] The aim was to identify the antitumor compounds of the fermentation broth of Paecilomyces militaris（strain RCEF0927）.[Methods] Anti-tumor activity was tested with a resazurin cytotoxicity assay model with Chinese hamster ovary（CHO） cells.Bioactive compounds were analysed with combined method of High Performance Liquid Chromatography（HPLC）,High Resolution Mass Spectrometer（HRMS） and antitumor assay.[Results] Antitumor test showed that the fermentation broth had strong cytotoxicity against CHO cells. Extraction tests showed that ethylacetate was the best solvent for thebioactive constituents extracting. HPLC-DAD- HRMS-Cytotoxicity assay revealed that the molecular formula of the active compounds in the extract were possibly C10 H13 N5 03, C22 H22 09 , C23 H24 010, C22 H22 O10, C15 Hlo 04, Cl5 H10 O, C36 H70 011 , C36 H68 O12 , C38 H74 Oll , C4o H76 O13 and C44 H82 014. [ Conclusion] The possible structures of the bioactive compounds were deduced with bioassay, HRMS, Uv and database inquiry as cordycepin, daizein, genistein 7,4′-dihydroxyisoflavone-7-O-（4＂-O-methyl）- b-D-glucopyranoside, 7, 4′-dihydroxy-6- methoxyisoflavone-7-O-（ 4＂-O-methyl）-b-D-glucopyranoside, 5, 7, 4′-trihydroxy- isoflavone-7- O- （4＂- O- methyl） -b-D-glucopyranoside, polyoxyethylene ether oleate, dehydro products of polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxypropylene or polyoxybutene contained derivants of polyoxyetbylene sorbitan fatty acid ester. Cytotoxicity of the compounds was revealed for the first time.