为了研究西洋菜是否可以应用于有重金属铜污染的富营养化地表水体的治理和生态修复中,以不同浓度的铜处理3d的西洋菜为实验材料,研究了在短期的培养条件下铜对西洋菜生长的影响。主要测定了抗氧化系统的几种酶,包括POD、CAT、SOD的活性以及可溶性蛋白、叶绿体色素含量的变化。研究表明,浓度为0.05mg·L^-1左右的Cu^2+对西洋菜的生长有一定的促进作用,此时西洋菜利用光能等的能力均有一定的提高;浓度为0.5~5mg·L^-1的Cu^2+对西洋菜的生长造成一定的环境胁迫,此时西洋菜保护酶活性明显提高;浓度大于5mg·L^-1的Cu^2+对西洋菜造成严重的过氧化损伤,此时保护酶的调节能力降低。西洋菜对铜的耐受能力不高,只适合应用于铜浓度极低的富营养化水体的治理和生态修复。
Nasturtium officinale R.Br. was cultivated in Steinberg medium with different concentration of Cu^2+ for 3 days to study the effect of Cu^2+ on the growth of Nasturtium officinale R.Br., in order to investigate if this kind of plant can be used in restoring eutrophicated water with copper pollution. Chlorophyll content, soluble protein content, POD activity, CAT activity, SOD activity were measured The result showed that Nasturtium officinale R.Br. could grow better in low copper concentration water compared with that of control. Light usage ability of Nasturtium officinale R.Br. increased in the medium with about 0.05 mg· L^-1 Cu^2+, while, Nasturtium officinale R.Br. suffered oxidative stress in medium of Cu^2+ concentration from 0.5 to 5 mg·L^-1, with protective enzymes increasing greatly. Nasturtium officinale R.Br. underwent seriously oxidative stress when Cu^2+ concentration was higher than 5 mg·L^-1 in waterbody. Regulation abilities of protective enzymes decreased when Cu^2+ concentration was higher than 5 mg·L^-1 in waterbody. Since Nasturtium officinale R.Br. can not grow well in higher copper concentration water, it can only be used in eutrophicated water restoration with very low copper pollution.