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中文摘要：
本实验分别选用武汉东湖中营养和富营养湖区的湖水和底泥，并在水柱中添加氮或磷以设置高营养、中营养、中营养添加磷、中营养添加氮等四种营养环境测定这四种营养条件下栽培苦草（Vallisneria natans）的生长和生化指标变化，探讨不同营养环境对苦草生长的影响机制．实验结果表明，苦草的生物量、叶数和新芽数等生长指标在中营养环境最高，中营养环境添加磷次之，中营养环境添加氮较低，在高营养环境最低；苦草可溶性糖和游离氨基酸含量在高营养环境中最高，在中营养环境、中营养环境添加磷和中营养环境添加氮等处理间没有明显差异结果分析表明，高营养环境影响苦草的碳氮代谢水平并抑制苦草生长，这可能是由于苦草过量富集高营养环境中的氮素造成的；中营养环境中氮的升高会在一定程度上抑制苦草的生长，而磷的升高对苦草生长没有明显抑制作用．
英文摘要：
In order to understand the effects of water and sediment on the growth of submersed macrophyte during eutrophication of waterbody, an experiment was carried out to study the effects of water and sediment from mesotrophic and eutrophic area of Lake Donghu on the growth of Vallisneria natans. Four treatments were designed, including eutrophic and mesotrophic culture conditions, and mesotrophic condition with inorganic nitrogen and phosphorus addition respectively. Indices of the growth experiments included biomass, both the number and length of leaves, number of sprout, were measured. Biochemistry indices including soluble carbohydrate content and free amino acid content were measured. The results showed that biomass, number of leaves and stolen of E natans were the highest growing in mesotrophic condition, phosphorus addition was the second, and nitrogen addition was the third. The plant in eutrophic condition showed the lowest growth. The results indicted that high trophic condition inhibit the growth of V. natans significantly. Increase of inorganic nitrogen in mesotrophic water inhibited the plant more than did the increase of the phosphorus, suggesting that nitrogen enrichment during eutrophication may be more important than phosphorus for the decline of macrophytes. Soluble carbohydrate and free amino acid contents of E natans in eutrophic condition were significantly higher than those in mesotrophic conditions, indicating that carbon and nitrogen metabolism of the plant were affected. This might be attributed to excessive accumulation of N in eutrophic waters of submersed macrophyte.
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