中文摘要：

目的：建立一种操作简单、可行的大鼠异位心肺联合移植模型，以利于移植排斥反应机制的研究及其实验性治疗研究。方法：分离受体Wistar大鼠颈部并形成皮囊，提供容受供体心肺的空间，分离颈总动脉。供体经过肝素化后，在0～4℃条件下取心肺，并作适当修剪，最后在0～4℃条件下将心肺供体SD大鼠的主动脉与受体Wistar大鼠的颈总动脉行端侧吻合，并进行心肺复苏。结果：心肺联合移植正式手术50例，手术后通血复跳率96％。手术时间为（70±10）min，平均冷缺血时间为（40±10）min。手术主要并发症是动脉吻合口狭窄及出血，血栓形成，失血性休克。未作任何处理的移植心肺3天存活率82％。结论：成功建立了大鼠颈部心、肺联合移植模型。

英文摘要：

Objective： To establish a simplified and useful heterotopy heart-lung transplantation rat model for studying of the mechanism of transplantation rejection and cure the rejection in animal model. Methods： The heterotopic cervical heart-lung transplantation model was established by anastomosing the aorta of the SD rat heart to the right common carotid of the Wistar rat. A total of 50 couples allogene rats were performed same proceeding like that. Results ： The rate of successful beat of the donors＇ heart was 96%. The average operative time and graft ischemia time were （ 70 ± 10） min and （40 ± 10） min respectively. The main complications included hemorrhage and stenosis of anastomosing stoma, thrombosis and hemorrhagic shock. The 3 days survival rate of heart-lung allograft without any treatment was 82%. Conclusion： A simplified and useful rat heterotopic heart-lung transplantation model was established successfully.