中文摘要：

目的探讨小分子免疫抑制药J2抑制小鼠皮肤移植排斥反应的作用。方法以C57BL／6小鼠为供体、Balb／c小鼠为受体建立皮肤移植模型，将模型小鼠随机分为A组、B组、c组，每组15只，并分别给予空白液、环孢素A（10mg·kg^-1）和J2（30mg·kg^-1）灌胃，均每天给药1次，连续12d，比较各组皮瓣的存活时间和存活率，术后14d进行组织学检查。结果A组发生排斥反应的时间延迟，皮瓣平均存活时间为（13.7±1.4）d；B组皮瓣平均存活时间[（18，2±2，1）d]与C组皮瓣平均存活时间[（17，7±2．4）d]均较A组明显延长（均P〈0．05）。B组与C组平均皮瓣存活时间、存活率均差异无显著性（均P〉0．05）。组织学检查证实，C组术后14d植片未见明显淋巴细胞浸润。结论J2可明显抑制小鼠皮肤移植排斥反应。

英文摘要：

Objective To study the effect of a small - molecule compound J2 in the inhibition of skin allograft rejection in mice. Methods C57BL/6 and Balb/c mice were used as donors and recipients respectively for the establishment of a skin allagraft model. 45 mice with the model were then randomly divided into 3 equal groups. Mice of group A, B and C were given each placebo, 10 mg· kg^-1 of cyclosporin A and 30 mg·kg^-1 of J2 administered by gastrogavage q. d. , respectively. The course of treatment in the 3 groups lasted 12 days beginning at the day of transplantation. The graft survival time in mice of the different groups was assessed with the Kaplan-Meier analysis. 14 days after the transplantation, histological examination of the skin graft was carried out. Results The average graft survival time in mice of allograft control （group A） was （ 13.7 ± 1.4） d. The average graft survival tomes in mice of group B treated with cyclosporin A and mice of group C treated with J2 were （ 18.2 ± 2.1 ） d and （17.7 ±2.4） d, respectively, both of which being significantly longer than that of group A （P 〈0.01 ）. The difference between the average graft survival times in mice of group B and C, however, was insignificant （P〉0.05 ）. Histological examination demonstrated that no apparent lymphocyte infiltration could be found in the skin graft of mice treated with J2 （group C）. Conclusion These results show that J2 can inhibit allograft rejection dramatically.