中文摘要：

目的：检测小鼠角膜移植后核转录因子KB（NF-kB）活性变化。方法：建立以C57BL／6小鼠为供体，以BALB／c小鼠为受体角膜移植实验模型，随机分为A、B、C三组，A组为正常小鼠组，B组为自体角膜移植作为对照组，C组同种异基因角膜移植作为实验组，ELASA方法检测小鼠角膜移植后3d、5d、7d角膜NF-kB的活性变化。结果：组织学检查证实，同种异基因小鼠角膜移植后14d见明显淋巴细胞浸润。3d、5d角膜NF-kB的活性升高，7d达到高峰，与原位移植组有显著差异（P〈0．01）。结论：小鼠同种异基因角膜移植后，角膜NF-kB的活性升高，可能参与移植排斥。

英文摘要：

Objeetlve：To determine the expression of NF-kB activation species of grafts in the early pre-rejection postoperative period after corneal transplantation. Methods： The donor and acceptor corneas of two groups of mice were studied after receiving an allo- （ C57BL/10 to BALB/c） or autograft （BALB/c）. Autograft was treated as control group. Graft rejection was observed by blomicroscopy. Grafted eyes were removed at specified times and examined histologically. NF-kB activation was assayed using ELISA-based transactivation TransAM kit. Results：Corneal rejection was characterized by opaque corneas with prominent neovascularization. NF-kB activation in cornea was significantly increased compared the to autograft following corneal transplantation. Conclusion：The results indicate that NF-kB activation increases in allograft corneal.