欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
基于多壁碳纳米管/纳米Ag-TiO2复合膜制备了高灵敏度的DNA电化学生物传感器。将Ag-TiO2复合物与适量分散于N,N-二甲基甲酰胺中的多壁碳纳米管（MWNT）相混合,形成均匀稳定的混合溶液,将其滴涂于裸碳糊电极表面,制得MWNT/Ag-TiO2修饰碳糊电极。碳纳米管大的比表面积和良好的电子传递性能与Ag-TiO2纳米复合物良好的生物相容性和对DNA极好的吸附能力的协同作用,显著提高了DNA探针的固载和DNA杂交的检测灵敏度。应用循环伏安法和电化学交流阻抗谱分别对传感膜的制备和DNA的固定与杂交进行了表征。以电化学交流阻抗谱法对转基因植物外源草丁膦乙酰转移酶基因片段进行了检测,线性范围为1.0×10-11～1.0×10-6mol/L,检出限为3.12×10-12mol/L。
英文摘要：
A highly sensitive electrochemical deoxyribonucleic acid（ DNA） biosensor based on multi-walled carbon nanotubes（ MWNT） /Ag-TiO2 composite film was developed. The solution containing Ag-TiO2-MWNT composite was casted on the carbon paste electrode surface to form a robust film,which combine the advantages of the good biocompatibility of Ag-TiO2 naocomposite and the fine conductivity,as well as the large active surface area of carbon nanotubes. The composite could greatly improve the immobilization capacity of the probe DNA. The morphologies and electrochemistry of the nanocomposite film were investigated by scanning electron microscopy and electrochemical techniques including electrochemical impedance spectroscopy and cyclic voltammetry,respectively. DNA hybridization events were monitored by a label-free method of electrochemical impedance spectroscopy. This label-free electrochemical impedance DNA biosensor showed high sensitivity and selectivity for phosphinothricin acetyltransferase gene sequence assay. The multicomponents films also displayed a high stability during repeated regeneration and hybridization process.
同期刊论文项目
同项目期刊论文
Ultrasensitive indicator-free and enhanced self-signal nanohybrid DNA sensing platform based on elec
Novel fabrication of single-stranded DNA/graphene/polyaniline nanocomplex for label-free voltammetri
Synergistic Effect of MWNTs/CeO2/CHIT Film for Detection of CdSe Nanoparticle Labeled Sequence-speci
Sensitively Electrochemical Sensing for Sequence-Specific Detection of Phosphinothricin Acetyltransf
Electrochemical Characterization of (ZnO/dsDNA)(n) Layer-by-layer Films and Detection of Natural DNA
Electrochemical Study of Lincomycin on Au-PtNPs/nanoPAN/Chitosan Nanocomposite Membrane and Its Dete
Electrochemical Deoxyribonucleic Acid Biosensor Based on Multiwalled Carbon Nanotubes/Ag-TiO2 Compos
Controllable fabrication of Au micro/nanostructures on self-doped polyaniline nanofibers via electro
Synergistic membrane of ZrO2/self-doped polyaniline nanofibres fabricated by controllable electrodep
Ionic liquid-functionalized graphene as modifier for electrochemical and electrocatalytic improvemen
Electroosmotic flow-switchable poly (dimethylsiloxane) microfluidic channel modified with cysteine b
Application of cadmium sulfide nanoparticles as oligonucleotide labels for the electrochemical detec
Electrocatalytic oxidation of dopamine at an ionic liquid modified carbon paste electrode and its an
A DNA electrochemical sensor prepared by electrodepositing zirconia on composite films of single-wal
Direct electrochemistry and electrocatalysis of hemoglobin in sodium alginate film on a BMIMPF6 modi
Near infrared sensing based on fluorescence resonance energy transfer between Mn:CdTe quantum dots a
Voltammetric investigation on interaction of protein with chromotrope 2R and its analytical applicat
Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analys
An ionic liquid supported CeO2 nanoshuttles-carbon nanotubes composite as a platform for impedance D
A Novel Hydrogen Peroxide Biosensor Based on the Synergistic Effect of Gold-Platinum Alloy Nanoparti
Electrochemical characterization of deoxyribonucleic acid on aluminum (III)/poly(L-glutamic acid) fi
Electrochemical immunosensor for simultaneous detection of dual cardiac aarkers based on PDMS-Gold n
Tree-shaped paper strip for semiquantitative colorimetric detection of protein with self-calibration
Quantum dots electrochemical aptasensor based on three-dimensionally ordered macroporous gold film f
An electrochemical biosensor based on Nafion-ionic liquid and a myoglobin-modified carbon paste elec
Highly Sensitive Indicator-Free Impedance Sensing of DNA Hybridization Based on Poly(m-aminobenzenes
Selective Synthesis and Luminescence Properties of Self-Assembled SrMoO4 Superstructures via a Facil
Rapid DNA electrochemical biosensing platform for label-free potentiometric detection of DNA hybridi
PDMS gold nanoparticle composite film-based silver enhanced colorimetric detection of cardiac tropon
Immobilization-free direct electrochemical detection for DNA specific sequences based on electrochem
Direct electrochemistry and electrocatalysis of hemoglobin on gold nanoparticle decorated carbon ion
Label-free amperometric immunobiosensor based on a gold colloid and Prussian blue nanocomposite film
Electrochemical DNA biosensor based on chitosan/nano-V2O5/MWCNTs composite film modified carbon ioni
Direct electrochemistry and electrocatalysis of hemoglobin based on poly(diallyldimethylammonium chl
Green synthesis and potential application of low-toxic Mn : ZnSe/ZnS core/shell luminescent nanocrys
Three-dimensional Dendritic Pt Nanostructures: Sonoelectrochemical Synthesis and Electrochemical App
Improved hydrostatic pressure sample injection by tilting the microchip towards the disposable minia
Direct electron transfer of hemoglobin in layered alpha-zirconium phosphate with a high thermal stab
Electropolymerization of high stable poly(3,4-ethylenedioxythiophene) in ionic liquids and its poten
Synthesis, characterization and computational study of nitrogen-doped CeO2 nanoparticles with visibl
Rapid and cost-effective detection of sequence-specific DNA by monitoring the electrochemical respon
Direct electrocatalytic oxidation of adenine and guanine on carbon ionic liquid electrode and the si
Highly sensitive and selective cholesterol biosensor based on direct electron transfer of hemoglobin
Synergistic effects of nano-ZnO/multi-walled carbon nanotubes/chitosan nanocomposite membrane for th
A DNA electrochemical sensor with poly-L-lysine/single-walled carbon nanotubes films and its applica
A PDDA/poly(2,6-pyridinedicarboxylic acid)-CNTs composite film DNA electrochemical sensor and its ap
Sensitive PAT gene sequence detection by nano-SiO2/p-aminothiophenol self-assembled films DNA electr
Hydrothermal synthesis of monodisperse Ag2Se nanoparticles in the presence of PVP and KI and their a
CdTe quantum dots as probes for near-infrared fluorescence biosensing using biocatalytic growth of A
Direct electron transfer of hemoglobin in a CdS nanorods and Nafion composite film on carbon ionic l
Hemoglobin-CdTe-CaCO3@Polyelectrolytes 3D Architecture: Fabrication, Characterization, and Applicati
Enhanced sensitivity for deoxyribonucleic acid electrochemical impedance sensor: Gold nanoparticle/p
Electrochemical biosensor for the detection of cauliflower mosaic virus 35 S gene sequences using le
Cadmium(II) (8-hydroxyquinoline) chloride nanowires: Synthesis, characterization and glucose-sensing
Ionic-liquid-doped polyaniline inverse opals: preparation, characterization, and application for the
Preparation and bioapplication of high-quality, water-soluble, biocompatible, and near-infrared-emit
Label-Free Colorimetric Detection of Small Molecules Utilizing DNA Oligonucleotides and Silver Nanop
Plasmonic Cu2-xS nanocrystals: Optical and structural properties of copper-deficient copper(I) sulfi
Electrochemistry and electrocatalysis of hemoglobin on multi-walled carbon nanotubes modified carbon
Synthesis of gelatin-stabilized gold nanoparticles and assembly of carboxylic single-walled carbon n
Enhanced electrochemiluminescence of CdSe quantum dots composited with CNTs and PDDA for sensitive i
Electrochemical behaviors of metol on hydrophilic ionic liquid 1-ethyl-3-methylimidazolium tetrafluo
Colorimetric detection of mercury ion (Hg2+) based on DNA oligonucleotides and unmodified gold nanop
Electrodeposition of Co Nanoparticles on the Carbon Ionic Liquid Electrode as a Platform for Myoglob
Myoglobin/gold nanoparticles/carbon spheres 3-d architecture for the fabrication of a novel biosenso
One-pot template-free fabrication of hollow magnetite nanospheres and their application as potential
DNA aptamer-based detection of lysozyme by an electrochemiluminescence assay coupled to quantum dots
Highly sensitive electrochemical impedance spectroscopic detection of DNA hybridization based on Au-
Analysis of nonadherent apoptotic cells by a quantum dots probe in a microfluidic device for drug sc
Direct electrochemistry of guanosine on multi-walled carbon nanotubes modified carbon ionic liquid e
Synergistically improved sensitivity for the detection of specific DNA sequences using polyaniline n
Electrochemical sensing the DNA damage in situ induced by a cathodic process based on Fe@Fe2O3 core-
Electrochemical synthesis for flowerlike and fusiform christmas-tree-like cerium hexacyanoferrate(II
Conductive architecture of Fe2O3 microspheres/self-doped polyaniline nanofibers on carbon ionic liqu
Readily Reusable Electrochemical DNA Hybridization Biosensor Based on the Interaction of DNA with Si
Electrodeposition of Prussian Blue Nanoparticles on Electrochemically Reduced Graphene Oxide and Syn
Fabrication of DNA/graphene/polyaniline nanocomplex for label-free voltammetric detection of DNA hyb
Conjugated self-doped polyaniline-DNA hybrid as trigger for highly sensitive reagentless and electro
Electrochemical sensing the genotoxicity of Cu2O nanocubes activated by hydrogen peroxide electro-ge
Improved electrochemical performances of polyaniline nanotubes-poly-L-lysine composite for label-fre
Highly sensitive electrochemical impedance sensing of PEP gene based on integrated Au-Pt alloy nanop
Electrochemically reduced graphene oxide-enhanced electropolymerization of poly-xanthurenic acid for
Freely switchable impedimetric detection of target gene sequence based on synergistic effect of ERGN
期刊信息
位置：