欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
tRNaseZ是一种参与tRNA前体3’末端加工的核酸内切酶，根据氨基酸残基数目分为长型（tRNaseZ-L）和短型（tRNase Z-8）两类．细菌只有短型，而真核生物既可以有短型也可以有长型，并且绝大部分真核生物只有一个长型．为了研究短型tRNaseZ和长型tRNaseZ的功能保守性，分别构建表达编码枯草芽孢杆菌（Bacillussubtilis）短型tRNaseZfBsuTrzl）、人短型tRNaseZ（ELACl）以及海栖热袍菌（Thermotoga maritima）短型（RNaseZfTmaTrzl）的酵母低表达载体，并转化tRNaseZ-1温敏型粟酒裂殖酵母（Schizosaccharomycespombe）进行互补救活实验．结果显示，与粟酒裂殖酵母tRNaseZ-L（SpTrzl）相比，ELACl能在37℃完全救活酵母tRNaseZ-L温敏型菌株，而BsuTrzl的救活能力略弱．但具有不同底物特异性的TmaTrzl则完全不能救活．此外．催化活性受损的ELACl组氨酸模体突变体（ELACl．H62A和ELACl—H64A）救活酵母tRNaseZ。温敏型菌株的能力极大受损．这些结果表明，枯草芽孢杆菌和人的tRNaseZ0能替代粟酒裂殖酵母的tRNaseZ-L，并且这些蛋白具有功能保守性．这些实验进一步支持了长型tRNaseZ基因是由短型tRNaseZ基因复制后序列进化形成的观点．
英文摘要：
tRNase Z is the tRNA 3＇-end processing endonuclease that removes the 3＇-trailer sequences from precursor tRNAs. Based on the size of the amino acid sequence, tRNase Z can be divided in short （tRNase Zs） and long （tRNase ZL） forms. Bacteria has only tRNase Zs, whereas eukaryotes can have both tRNase Zs and tRNase ZL and the majority of eukaryotes have one tRNase ZL. To study the functional conservation be- tween tRNase Zs and tRNase ZL, we constructed the expression vectors for tRNase Zs from Bacillus subtilis （BsuTrzl）, Thermotoga maritima （TmaTrzl） and humans （ELAC1） and transformed them into a tRNase ZL- temperature sensitivity mutant strain of the fission yeast Schizosaccharomyces pombe. Compared to S. pombe tRNase ZL （SpTrzl）, ELAC1 and, to a lesser extant, BsuTrzl could restore the normal growth of the mutant strain at 37 ℃, whereas TmaTrzl, which has the different substrate specificity, could not rescue the tempera- ture sensitive mutant. In addition, the ability of catalytically inactive mutants of ELAC1 （ELAC1-H62A and ELAC1-H64A） to rescue the temperature sensitive mutant was compromised. These results suggest that BsuTrzl and ELAC1 can substitute for SpTrzl and that these proteins are functionally conserved. These re- suits also support the idea that tRNase ZL arises from gene duplication of tRNase Zs and further sequence di- vergence.
同期刊论文项目
同项目期刊论文
Analysis of gene expression changes, caused by exposure to nitrite, in metabolic and antioxidant enz
Differences in growth, pigment composition and photosynthetic rates of two phenotypes Microcystis ae
Photosynthetic changes of flag leaves during senescence stage in super high-yield hybrid rice LYPJ g
Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haloduran
Synthesis and evaluation of a novel beta-cyclodextrin derivative for oral insulin delivery and absor
The complete mitochondrial genome of the hybrid of Pelteobagrus fulvidraco (female symbol) x Pelteob
TPGS2k/PLGA nanoparticles for overcoming multidrug resistance by interfering mitochondria of human a
Involvement of Relish gene from Macrobrachium rosenbergii in the expression of anti-microbial peptid
Efficient glycosylation of puerarin by an organic solvent-tolerant strain of Lysinibacillus fusiform
Resveratrol prevents cadmium activation of Erk1/2 and JNK pathways from neuronal cell death via prot
A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression
Identification, characterization, and functional studies of a Pelle gene in the Chinese mitten crab,
Inducible HSP70 antagonizes IL-1beta cytocidal effects through inhibiting NF-kB activation via desta
The complete mitochondrial genome of Pauropus longiramus (Myriapoda: Pauropoda): implications on ear
Molecular cloning of manganese superoxide dismutase gene in the cladoceran Daphnia magna: effects of
Inhibition of inflammatory mediators contributes to the anti-inflammatory activity of KYKZL-1 via MA
Molecular structure, expression pattern and functional characterisation of APRIL in an aquatic mamma
Growth inhibitory effect of KYKZL-1 on Hep G2 cells via inhibition of AA metabolites and caspase-3 p
Physiological and growth characteristics of Ginkgo biloba L. exposed to open ？eld and shade enclosur
Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis pota
Development of 39 novel polymorphic microsatellite markers for the giant mottled eel Anguilla marmor
Development and validation of RP-HPLC method for the simultaneous quantification of seven flavonoids
Liquid laccase production by Phomopsis iquidambari B3 accelerated phenolic acids degradation in long
Differences in long-term impacts of un-ionized ammonia on life-history traits of three species of Da
Morphological, physiological and biochemical responses of biofuel plant Euphorbia lathyris to salt s
Baicalein reduces lipopolysaccharide-induced in？ammation via suppressing JAK/STAT sactivation and RO
Molecular characterization, expression and binding activity of the cytokines TNFSF14 and its recepto
Characterization of polysaccharides with marked inhibitory effect on lipid accumulation in Pleurotus
Copper/zinc superoxide dismutase from the Cladoceran Daphnia magna: molecular cloning and expression
Sensitive and reliable multianalyte quantitation of herbal medicine in rat plasma using dynamic trig
Deep sequencing of small RNA repertoires in mice reveals metabolic disorders-associated hepatic miRN
Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Erio
Does microcystin disrupt the induced effect of Daphnia kairomone on colony formation in Scenedesmus?
Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main
Metabolic and antioxidant responses in juveniles of Cherax quandricarinatus under actute cadmium str
The superoxide dismutase from red claw crayfish, Cherax quadricarinatus: molecular cloning and chara
Tunicamycin inhibits PDGF-BB-induced proliferation and migration of vascular smooth muscle cells thr
Cloning and characterization of two different L-type lectin genes from the Chinese mitten crab Erioc
期刊信息
位置：