欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
该研究以洋桔梗（Eustoma grandi florum）2个品种‘玛丽艾基粉色’和‘圣剑白底紫边’为试材,提取叶片DNA,经过EcoRI /Mse Ⅰ双酶切、连接、预扩增、选择性扩增,建立了洋桔梗的AFLP最佳反应体系;并以64个常用引物组合进行扩增,得到154个多态性条带,从中筛选出扩增条带较多且多态性较好的4个引物组合（E-ACA/M-CTC,E-ACC/M-CAC,E-AGC/M-CTT,E-ACT/M-CAG）,其多态位点百分率均值为24.36％.利用上述4个引物组合,以最佳反应体系为基础,构建了7个常见洋桔梗品种的AFLP指纹图谱,统计7个品种各4个引物组合在1 000～300 bp区间7个区段的扩增条带,并将各个品种的AFLP指纹图谱转换成各品种4组7位数构成的28位特异数字指纹,极大地方便了种质比较及鉴定;7个品种间的遗传相似系数介于0.683 5～0.860 8之间,平均值为0.774 6.研究结果为进一步进行洋桔梗的种质研究及利用奠定了基础.
英文摘要：
Taking Eustoma grandiflorum cultivars ‘Double Mariachi Pink’ and ‘Excalibur Blue Picotee’ as experimental material,we extracted genomic DNA from the leaves.The optimized AFLP reaction system was established,after EcoR Ⅰ/Mse Ⅰ double enzyme digestion,ligation,pre amplification and selective am plification.154 polymorphic bands were acquired with 64 primer combinations commonly used,from which 4 selective amplification primer combinations （E-ACA/M-CTC,E-ACC/M-CAC,E-AGC/M-CTT,E-ACT/ M-CAG） with more polymorphic amplified bands were selected.The average percentage of polymorphic bands was 24.36 ％.Using the 4 selective amplification primer combinations,through the optimized reaction system,the AFLP fingerprints of 7 common E.grandiflorum cultivars were constructed.Subsequently,the AFLP fingerprints were transformed into the specific digital fingerprints of 28 digits,based on the counting of the amplified bands in the 7 sections of 1 000～300 bp interval of the 4 primer combinations,greatly fa cilitating the comparison and identification of the germplasm.The genetic similarity coefficient between the cultivars ranged 0.683 5～0.860 8,and the average value was 0.774 6.The results of that study have laid an important foundation for the further research on E.grandiflorum.
同期刊论文项目
同项目期刊论文
Analysis of gene expression changes, caused by exposure to nitrite, in metabolic and antioxidant enz
Differences in growth, pigment composition and photosynthetic rates of two phenotypes Microcystis ae
Photosynthetic changes of flag leaves during senescence stage in super high-yield hybrid rice LYPJ g
Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haloduran
Synthesis and evaluation of a novel beta-cyclodextrin derivative for oral insulin delivery and absor
The complete mitochondrial genome of the hybrid of Pelteobagrus fulvidraco (female symbol) x Pelteob
TPGS2k/PLGA nanoparticles for overcoming multidrug resistance by interfering mitochondria of human a
Involvement of Relish gene from Macrobrachium rosenbergii in the expression of anti-microbial peptid
Efficient glycosylation of puerarin by an organic solvent-tolerant strain of Lysinibacillus fusiform
Resveratrol prevents cadmium activation of Erk1/2 and JNK pathways from neuronal cell death via prot
A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression
Identification, characterization, and functional studies of a Pelle gene in the Chinese mitten crab,
Inducible HSP70 antagonizes IL-1beta cytocidal effects through inhibiting NF-kB activation via desta
The complete mitochondrial genome of Pauropus longiramus (Myriapoda: Pauropoda): implications on ear
Molecular cloning of manganese superoxide dismutase gene in the cladoceran Daphnia magna: effects of
Inhibition of inflammatory mediators contributes to the anti-inflammatory activity of KYKZL-1 via MA
Molecular structure, expression pattern and functional characterisation of APRIL in an aquatic mamma
Growth inhibitory effect of KYKZL-1 on Hep G2 cells via inhibition of AA metabolites and caspase-3 p
Physiological and growth characteristics of Ginkgo biloba L. exposed to open ？eld and shade enclosur
Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis pota
Development of 39 novel polymorphic microsatellite markers for the giant mottled eel Anguilla marmor
Development and validation of RP-HPLC method for the simultaneous quantification of seven flavonoids
Liquid laccase production by Phomopsis iquidambari B3 accelerated phenolic acids degradation in long
Differences in long-term impacts of un-ionized ammonia on life-history traits of three species of Da
Morphological, physiological and biochemical responses of biofuel plant Euphorbia lathyris to salt s
Baicalein reduces lipopolysaccharide-induced in？ammation via suppressing JAK/STAT sactivation and RO
Molecular characterization, expression and binding activity of the cytokines TNFSF14 and its recepto
Characterization of polysaccharides with marked inhibitory effect on lipid accumulation in Pleurotus
Copper/zinc superoxide dismutase from the Cladoceran Daphnia magna: molecular cloning and expression
Sensitive and reliable multianalyte quantitation of herbal medicine in rat plasma using dynamic trig
Deep sequencing of small RNA repertoires in mice reveals metabolic disorders-associated hepatic miRN
Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Erio
Does microcystin disrupt the induced effect of Daphnia kairomone on colony formation in Scenedesmus?
Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main
Metabolic and antioxidant responses in juveniles of Cherax quandricarinatus under actute cadmium str
The superoxide dismutase from red claw crayfish, Cherax quadricarinatus: molecular cloning and chara
Tunicamycin inhibits PDGF-BB-induced proliferation and migration of vascular smooth muscle cells thr
Cloning and characterization of two different L-type lectin genes from the Chinese mitten crab Erioc
期刊信息
位置：