中文摘要：

目的：通过观察益气活血法中药对脑出血大鼠脑损伤区组织促血管生成素-1（Ang-1）及其受体〔为含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶-2（Tie-2）〕表达的影响,探讨益气活血法治疗脑出血的作用机制。方法：288只SD大鼠被随机分为正常组8只,假手术组、模型组、益气活血组、益气组和活血组各56只。采用型胶原酶诱导脑出血大鼠模型。相应灌服益气活血法代表方补阳还五汤全方及其益气组分、活血组分药物（剂量均为临床70 kg成人用量的3倍）。各组于术后1、2、4、7、14、21和28 d各取8只大鼠,采用苏木素-伊红（HE）染色观察大鼠脑组织形态学改变,用免疫组化法检测Ang-1及其受体Tie-2的表达,计数阳性血管作为观察指标。结果：HE染色显示正常组及假手术组各时间点未见血肿及局部明显病理学改变,而益气活血组微血管段多于活血组、益气组和模型组。免疫组化研究显示正常组及假手术组不同时间点Ang-1和Tie-2表达均未见明显变化;模型组、益气组、活血组及益气活血组脑出血后1 d即有Ang-1和Tie-2表达,模型组与益气组至21 d达到高峰,随后开始下降,活血组及益气活血组表达高峰时间提前至14 d,至21 d仍维持一定的表达水平。结论：在脑出血微血管系统重建过程中,益气活血法可能通过强化血肿周围Ang-1和受体Tie-2的表达,进而促进脑出血损伤区血管新生的作用,促进脑出血大鼠神经功能的恢复。

英文摘要：

Objective： To investigate the mechanisms of method of supplementing Qi and activating blood circulation （益气活血法） by observing its effects on expressions of angiopoietin - 1 （Ang - 1） and the receptor tyrosine kinase that contains immunoglobulin - like loops and epidermal growth factor - similar domains- 2 （Tie - 2） in brains of rats with intraeerebral hemorrhage （ICH）. Methods： ICH model was induced by injecting collagenase type Ⅵ stereotaxically into right globus pallidus. Two hundred and eighty-eight SD rats were randomly divided into six groups, including normal control group （n = 8）, sham operation group, ICH model group, method of supplementing Qi and activating blood circulation - treated group, supplementing Qi -treated group and activating blood circulation- treated group （each n = 56）. The last 3 treated groups were administered orally with the classic method of supplementing Qi and activating blood circulation prescription of Buyang Huanwu decoction （补阳还五汤）, its supplementing Qi and activating blood circulation components, respectively, all at the dose of three times as a 70 kg human adult. The changes of brain tissues were observed by hematoxylin eosine （HE） staining, and the expressions of Ang - 1 and the receptor Tie- 2 were assayed by immunohistochemistry on 1, 2, 4, 7, 14, 21 and 28 days after the onset in 8 rats of each group, then positive microvessels were counted. Results： No hematoma and obvious local pathological changes appeared in brain tissues of normal or sham operation group at different time points during the experiment. Microvascular angiogenesis of method of supplementing Qi and activating blood circulation-treated group was more than that of supplementing Qi -treated group, activating blood circulation-treated group and model group. Immunohistochemical researches showed that at different time points the expressions of Ang - 1 and Tie - 2 had no significant changes in the normal control and sham operation groups. The expre