中文摘要：

目的 观察脑出血大鼠脑组织中基质金属蛋白酶-2（MMP-2）表达,探讨补阳还五汤治疗脑出血的作用机制。方法 采用立体定向技术制作大鼠脑出血模型,对出血后2、4、7、14、21、28 d后各组脑组织进行MMP-2免疫组织化学染色,检测脑组织各区域MMP-2的阳性微血管数。结果 正常组未见表达,假手术组皮质偶见MMP-2表达;模型组大鼠MMP-2呈双峰表达,在造模后4 d达最高峰（P〈0.01）,之后有所下降,至21 d仍有表达并再有小高峰出现（P〈0.01）;补阳还五汤组4 d时为表达低谷,并明显低于模型组（P〈0.01）,其后逐渐上升至高水平维持达2 w,其中7、14、21 d表达均高于模型组（P〈0.05）,28 d表达稍下降但仍高于模型组（P〈0.01）。结论 补阳还五汤可通过调节脑出血后MMP-2的表达,有利于微血管系统重建,改善出血后脑组织的微环境。

英文摘要：

Objective To investigate the mechanisms of Buyang Huanwu Decoction （BYHUD） by observing its effects on expressions of roatrix metalloproteinase-2 （MMP-2）in intracerebral hemorrhagic （ICH） rat brains. Methods ICH model was induced by injecting collagenase type Ⅶ stereotaxically into right globus pallidus. Sprague-Dawley rats were randomly divided into normal control group, sham operation group, model group and BYHUD-treated group. MMP-2 and MMP-2 positive microvessel count were detected on the 2nd, 4th, 7th, 14th,21th and 28th day after operation. The expressions of MMP-2 were evaluated by immunohistochemistry. Results Expression of MMP-2 was not observed in normal control group and observed occasionally in the cortex of sham operation group. In model group, the expression of MMP-2 was double hump and peaked on the 4th day （P 〈0. 01 ） ,then gradually decreased but peaked at 21th day （P 〈0. 01 ） again. In BY- HUD-treated group, expression of MMP-2 was lower than that in model group on the 4th day （P 〈 0. 01 ） , then increased and was higher than that in the model group during the 7th -21th day （P 〈0. 05）. It depressed on 28th day,but was still higher than that in the model group （P 〈 0. 01 ）. Conclusions BYHUD could regulate the expressions of MMP-2 in ICH rat brains, which might improve the microenvironment and promote the microvessel networks reconstruction after ICH.