欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的 探讨高表达三联组氨酸核苷酸结合蛋白2（Hint2）增强人乳腺癌细胞株MCF7对紫杉醇注射液敏感性的影响. 方法 构建高表达Hint2的载体pCMV-HA-Hint2并转染MCF7细胞, 通过免疫荧光和Western blot 方法检测Hint2的表达, 采用噻唑蓝（MTT）法检测高表达Hint2后MCF7细胞对紫杉醇注射液的敏感性, 采用流式细胞AnnexinV 方法 检测细胞凋亡. 结果 pCMV-HA-Hint2转染36 h后, 细胞的Hint2基因表达明显增加, 5 μmol.L-1的紫杉醇注射液处理24 h后, Hint2高表达组细胞活性为33.78%, 而对照组细胞活性为44.12%. 结论 Hint2高表达能显著增加MCF7细胞对紫杉醇注射液的敏感性.
英文摘要：
Objective To evaluate the effect of over-expression of Hint2 on sensitivity of breast cancer cell MCF7 to taxol . Methods The expression vector of pCMV-HA-Hint2 was constructed and transfected into MCF7 cells and the protein expression of Hint2 was detected by immunofluorescence and Western blotting. The changes of sensitivity to taxol after transfection were examined by MTF and flow cytometry respectively. Results In MCF7 cells, the protein levels of Hint2 were significantly increased after transfection, After exposed to 5 μmol · L^-1 taxol for 24 h, ceil viability of Hint2 over-expression group was 33. 78%, compared to 44.12% in control group. Conclusion Over-expression of Hint2 can enhance the sensitivity of MCF7 to taxol.
同期刊论文项目
同项目期刊论文
Down-regulation of p110 beta expression increases chemosensitivity of colon cancer cell lines to oxa
期刊信息
位置：