中文摘要：

研究西兰花中异硫氰酸盐（isothiocyanates，ITCS）诱导人胃腺癌SGC-7901细胞凋亡的作用及其机制．应用MTT法检测ITCS对SGC-7901细胞增殖的影响；流式细胞仪检测活性氧、线粒体膜电位（却。）和细胞凋亡率．实验证明ITCS可明显抑制SGC-7901细胞增殖；不同质量浓度的ITCS作用于SGC-7901细胞24h后，细胞内活性氧含量随给药剂量的增加而升高、线粒体膜电位依次降低．60、120、240μg／mL的ITCS作用于SGC-7901细胞48h后可见细胞凋亡率分别为9．1％、20．1％和55．4％．ITCS可通过刺激SGC-7901细胞内活性氧的产生，损伤肿瘤细胞线粒体，使其膜电位下降最终引起SGC-7901细胞凋亡．

英文摘要：

To investigate the pro-apoptosis effect of isothiocyanates （ITCS） on human gastric adenoma cells SGC -7901 and its mechanism. MTT assay was used to evaluate the influence of ITCS on cell proliferation. FCM was used to test intracellular ROS level, mitochondrial transmembrane potential （△ψm） and cell apoptotic rate in SGC -7901 cells. ITCS prominently inhibited proliferation of SGC -7901 cells. When treated with different concentration of ITCS for 24 h, ITCS could increase intracellular ROS level and decrease mitochondrial transmembrane potential in SGC -7901 cells; when treated with 60, 120, 240 μg/mL of ITCS for 48 h, cell apoptotic rates were 9.1%, 20.1% and 55.4% respectively. The result shows that ITCS increases intracellular ROS in SGC -7901 cells, which causes damnification of mitochondria, therefore, leads to apoptosis of SGC - 7901 cells.