中文摘要：

目的 获得衣原体GHC噬菌体衣壳蛋白Vp2基因及其蛋白.方法 提取噬菌体φCPG1及其核酸,用PCR技术扩增Vp2基因片段,再将其定位插入到原核表达载体pET30a（＋）中,构建重组表达质粒.然后将重组表达质粒转化入大肠杆菌（BL-21）中,并用酶切分析、PCR扩增及部分序列测定等方法对重组质粒进行了鉴定.然后诱导表达,用SDS-PAGE及蛋白印迹进行鉴定.结果 所获得的Vp2基因片段经测序,长度为561bp,检索确认其序列与Genebank一致.对重组质粒进行诱导表达,SDS-PAGE和蛋白质印迹均显示获得相对分子质量约32kDa的衣原体GPIC噬菌体衣壳蛋白Vp2.结论 成功表达了噬菌体衣壳蛋白Vp2,为进一步研究其作用机制和临床应用打下基础.

英文摘要：

Objective To clone and express chlamydial GPIC capsid Vp2 gene, and identify its protein. Methods The complete sequence of Vp2 gene from φCPG1 phage was amplified. The amplicor was cut by restriction endonuclease,linked to piasmid vector pET30a （ ＋ ）, and transformed into E. coil. The expression of recombinant Vp2 was identified by SDS-PAGE electrophoresis and Western blot. Results The recombinant 561bp gene was sequenced and proved to be q~CPGI Vp2 by searching Genebank. Expression of 32 kDa capsid was confirmed by SDS-PAGE and Western blot. Conclusion Chlamydial GPIC capsid Vp2 gene and protein were obtained, which is helpful for the next study.