中文摘要：

目的获得衣原体GPIC噬菌体衣壳蛋白Vp1基因及其蛋白。方法提取噬菌体φCPG1及其核酸，并对Vp1克隆、表达，鉴定。结果所获得的Vp1基因片段经测序，长度为1661bp，检索确认其序列与Genebank一致。对重组质粒进行诱导表达，SDS-PAGE和蛋白质印迹均显示获得相对分子质量约62000的衣原体GPIC噬菌体衣壳蛋白Vp1。结论成功获得了噬菌体衣壳蛋白Vp1。为进一步研究打下基础。

英文摘要：

Objective To clone, express, and identify chlamydial GPIC capsid Vpl gene and protein. Methods The complete sequence of Vpl gene from φCPG1 phage was amplified. The amplicor was cut by restriction endonuclease, linked to plasmid vector, and transformed into E. coli. The expressed protein of recombinant Vpl was purified and identified. Results The recombinant 1 661 bp gene was sequenced and proved to be φCPG1 Vpl by searching Genebank. A 62 kDa capsid protein was expressed and confirmed by SDS-PAGE and Western blot. Conclusion The recombinant Vpl seems to be a highly conserved and specific marker for chlamydial phage.