中文摘要：

以新疆雪莲叶片、叶柄和根为试验材料,研究了MS基本培养基附加不同质量浓度的植物激素对新疆雪莲愈伤组织诱导的影响。结果表明：单因素诱导中,NAA的诱导效果显著优于其它激素;以NAA为诱导激素,叶片与根,叶柄与根之间的诱导率存在极显著性差异,诱导效果：根〉叶柄〉叶片,最高诱导率分别为100%、98%、44%;NAA与6-BA激素组合对3种外植体愈伤组织的诱导中,叶片与叶柄诱导率之间存在极显著性差异,叶片、叶柄与根的诱导率之间存在显著性差异,诱导效果：叶片〉叶柄〉根,最高诱导率分别为98%、64%、23%。

英文摘要：

Using leaves,petioles and roots of Saussurea involucrate as explants,the effect of plant hormones on callus induction in MS medium was studied.The results showed that the medium containing NAA was better than other media.Comparing with whole explants,callus induction of leaves and roots,petioles and roots differed significantly.The inducing effect of roots,leaves and petioles was 100%,98% and 44%,respectively.Using the combination of NAA and 6-BA,callus induction of leaves,petioles and roots differs significantly as well.The induction effect of leaves,petioles and roots was 98%,64% and 23%,respectively.