制备出分裂相多、杂质少、背景清晰、染色体分散且形态好的染色体标本,一直是细胞遗传学实验课关注的重点和难点.本文在去壁低渗火焰干燥法的基础上,改良了小麦染色体制片方法,分析了影响染色体标本制备效果的主要因素.
It has been the focus and difficulty of cytogenetic experimental class that prepares good chromosome specimens with more division phases, less impurities, clearer background, chromosome dispersion and good morphological. In this paper, we improved methods of wheat chromosomes preparation on the basis of wall hypotonic flame drying method, and analyzed the main influencing factors of chromosome specimen preparation.