中文摘要：

以新疆阿魏菇为材料,研究菌龄、酶解时间、酶解液对阿魏菇菌丝原生质体产量的影响,同时还研究了不同渗透压稳定剂对原生质体再生的影响。结果显示,在0.6 mol/L KCl稳渗剂条件下,菌龄为8 d阿魏菇菌丝体（0.1 g）在0.8 mL混合酶解液中（1.5%离析酶＋0.5%纤维素酶＋2%溶菌酶）制备原生质体,35℃酶解3 h获得原生质体达5×106个/mL。原生质体再生结果显示,在0.8 mol/L蔗糖稳渗剂下,原生质体再生时间为16 d,其再生率可达0.6%。该文通过对阿魏菇原生质体分离和再生条件的研究,旨在从细胞水平上探索一条食用菌育种新途径,为发展和完善食用菌新菌株选育提供理论依据和技术借鉴。

英文摘要：

This paper focused on the preparation and regeneration of P.ferulaeLenzi protoplast.The 0.1 g mycelium was added into 0.8 mL enzyme solution containing 1.5 % segregation enzyme,0.5 % cellulase enzyme,2 % lysozyme and 0.6 mol/L KCl as osmotic stabitizer,which had been cultured in liquid PDA medium for 8 days.These mycelium was treated 3 h under 35 ℃ and the number of protoplast reached to 5×106.The frequency of protoplast regeneration can reach up to 0.6 % by cultured on PDA medium for 16 d with 0.8 mol/L sucrose as osmotic stabitizer.The purport of this paper was to explore a new way to edible fungi breeding at cellular level and provide the theoretical basis and technical reference for the development and improvement of edible fungi breeding.