中文摘要：

建立了高效液相色谱—二极管阵列检测器（HPLC-DAD）测定化妆品中肌肽含量的方法。样品在破乳剂存在的条件下,用纯水经超声波提取30 min,在3 000 r/min条件下离心10min,采用Prevail^TMCarbohydrate ES色谱柱（250 mm×4.6 mm,5μm）,以甲醇与乙酸水溶液（pH 3.5）作为流动相,以1.0 mL/min进行等度洗脱,检测波长为210 nm,柱温25℃。结果显示,肌肽在0.2~20.0 mg/L呈良好的线性关系,相关系数为0.999 9,保留时间和峰面积的相对标准偏差均小于0.5%,加标回收率为93.33%~108.64%,检出限为0.02mg/L,定量限为0.07mg/L。

英文摘要：

A method based on high performance liquid chromatograph-diode array detector （HPLC-DAD） for determination of carnosine in cosmetics was established. In the presence of demulsifier, the samples were extracted by water with the assistance of ultrasonic wave for 30 rain, and were separated by eentrifugation at 3 000 r/min for 10 min. PrevailTM Carbohydrate ES column（250 mm × 4.6 mm, 5 μm） was used with mobile phase of methanol-acetic acid （pH 3.5）, for isocratic elution with the flow rate of 1.0 mL/min. The detection wavelength was 210 nm, and the column temperature was 25 ℃. The results showed that, in the range of 0.2-20.0 mg/L, the linear correlation coefficient was 0.999 9. The relative standard deviations （RSD） of retention time and peak area were both less than 0.5%. The recovery rates were 93.33% -108.64%. The detection limit was 0.02 mg/L, and the limit of quantitation was 0.07 mg/L.