中文摘要：

建立了高效液相色谱测定贝类产品中软骨藻酸的检测方法,具体过程为：样品先经甲醇浸提后,再用甲醇-水（V（甲醇）∶ V（水）=1∶1）提取2次,合并提取液后用Zorbax SB300-C18柱（2．1 mm ×250 mm,5μm）进行分离,以乙腈-0．2%磷酸水溶液（含0．1%三乙胺）（ V（乙腈）∶V（磷酸水溶液）=12∶88）为流动相于室温下等度洗脱,流速为0．25 mL/min,进样量20μL,检测波长为242 nm.结果表明,在建立的提取方法和色谱条件下,软骨藻酸从复杂的样品基体中被分离出来,在0．02~1．0 mg/L质量浓度范围内具有良好的线性关系（r 〉0．999）,样品的平均加标回收率为108%,测量方法的 RSD 为3．5%（n =6）,检出限为23．5 ng/g.所建立的检测方法可适用于对贝类产品中软骨藻酸的快速检测.

英文摘要：

A rapid detection method by high performance liquid chromatography （ HPLC） was developed to determine the concentrations of domoic acid in different species of shellfish. After extraction by methanol and methanol-water （1∶1, V/V）, the sample was separated using a Zorbax SB300 -C18 chromatographic column （ 2. 1 mm × 250 mm, 5 μm ） , and was eluted with acetonitrile -0. 2% phosphoric acid solution （ containing 0. 5% triethylamine） （12∶88, V/V） at room temperature. The flow rate was set as 0. 25 mL/min. 20 μL of sample was individually injected, and the detection wavelength was 242 nm. The results showed that domoic acid can be well separated from the sample matrix within 8 min with a linear range of 0. 02 ~1. 0 mg/L （ r 〉 0. 999 ） . The average recovery as tested by adding standards was 108%, and the RSD was 3. 5% （n =6） . The detection limit estimated with three folds of standard deviations was 23. 5 ng/g. In con-clusion, the established method can be used to detect trace domoic acid in shellfish samples rapidly without further purification treatment by SPE columns.