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不同产地、不同生长年限人参SOD的比较
  • 期刊名称:中国医院药学杂志
  • 时间:0
  • 页码:994-996
  • 语言:中文
  • 分类:R93[医药卫生—生药学;医药卫生—药学]
  • 作者机构:[1]长春中医药大学中医药与生物工程研发中心,吉林长春130117
  • 相关基金:国家科技支撑计划(编号;2007BA138B02);国家自然科学基金项目(编号:30873371)
  • 相关项目:人参蛋白抗衰老机制研究
中文摘要:

目的:比较不同产地、不同生长年限人参SOD活力,为人参药材的质量评价和临床应用提供依据。方法:采用改良的邻苯三酚自氧化法测定各样品SOD活力,采用考马斯亮蓝法测定各样品蛋白含量,采用聚丙烯酰胺凝胶电泳及NBT显色法进行各样品酶活显示。结果:不同产地的人参SOD活力存在很大差别,最高为72.20U·mg^-1,最低为12.16U·mg^-1;4年和5年生人参SOD活力差别较小。结论:应用SOD活性分析方法可以对不同产地人参的质量进行快速、有效的评价。

英文摘要:

OBJECTIVE To compare the SOD activity of Panax ginseng from different ages and sources, and provide the hasis for quality evaluation and clinical application of ginseng herbs. METHODS Coomassie brilliant blue method was used to determine the protein concentration. Improved pyrogallol auto-oxidation method and NBT photoreduetion method were used to determine the SOD activity. RESULTS The SOD activitity of 15 places were different, respectively. The highest activity was 72. 20 U·mg^-1 ,The lowest activity was 12. 16 U·mg^-1. The SOD aetivitity of four years and five years ginseng could not be observed. CONCLUSION The analysis method was accurate, fast, thus it can be used to determinate the different sources of ginseng.

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