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人参生长发育过程中茎内5种酶活性的初步研究
  • ISSN号:1000-5684
  • 期刊名称:《吉林农业大学学报》
  • 时间:0
  • 分类:R284.1[医药卫生—中药学;医药卫生—中医学] S567.51[农业科学—中草药栽培;农业科学—作物学]
  • 作者机构:[1]长春中医药大学研发中心,长春130117
  • 相关基金:国家自然科学基金项目(30873371)
中文摘要:

以五年生人参茎为供试材料,分别于人参展叶期、开花期、结果期、果后参根生长期、枯萎期采集人参茎样品,采用中性缓冲液制备粗酶液,应用分光光度法测定硝酸还原酶(NR)、超氧化物歧化酶(SOD)、谷丙转氨酶(GPT)、葡萄糖磷酸异构酶(GP!)、延胡索酸酶(FUM)的活力。结果表明:人参生长发育过程中,茎内NR和FUM均在展叶期和开花期出现活力高峰,SOD、GPT、GPI的活力在结果期较高。

英文摘要:

Using five-year old ginseng stems as materials, changes of nitrae reductase (NR), superoxide dismutase (SOD), glutamic-pyruvic transaminase (GPT), glucose phosphate isomerase (GPI) and fumarase(FUM) activity of ginseng stems during growth and development were studied. Ginseng stem samples were collected respectively at the spreading stage, the flowering stage, fruit setting stage, tile root growing after fruit stage and the withering stage. Neutral buffer solution was adopted to extract the enzyme solution of radix ginseng. Spectrophotometry was used to test the activities of NR, SOD, GFF, GPI and FUM. The results showed that in the growth and development process of ginseng stems, the activity of NR and FUM have the peaks of the activity during the speading stage and the flowering stage. The activity of SOD, GPT and GPI during the fruit setting stage is higher than at other stages. Activity changes of 5 enzymes can provide a theoretical basis for the optimization of ginseng cultivation.

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期刊信息
  • 《吉林农业大学学报》
  • 中国科技核心期刊
  • 主管单位:吉林省教育厅
  • 主办单位:吉林农业大学
  • 主编:秦贵信
  • 地址:吉林省长春市新城大街2888号
  • 邮编:130118
  • 邮箱:jlndxb@vjlav.edu.cn
  • 电话:0431-84532914
  • 国际标准刊号:ISSN:1000-5684
  • 国内统一刊号:ISSN:22-1100/S
  • 邮发代号:
  • 获奖情况:
  • 全国优秀科技期刊,全国中文农业类核心期刊,中国科技论文统计源期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:16398