中文摘要：

目的：建立小鼠胚胎干细胞（embryonic stem cells,ES）的培养方法.方法：制备G418抗性的原代小鼠胚胎成纤维细胞,经丝裂霉素C处理后成滋养层细胞,将小鼠胚胎干细胞复苏后,应用含白血病抑制因子的ES细胞培养液,培养小鼠ES细胞,观察集落的生长情况,并在光镜下观察细胞形态.结果：小鼠胚胎成纤维细胞生长良好,ES细胞呈克隆状生长,且保持未分化状态.结论：建立了小鼠胚胎干细胞培养的有效方法,为下一步基因打靶奠定基础.

英文摘要：

Objective： To establish mouse embryonic stem ceils culture system and methods, Methods： Mitomycin Ctreated G418-resistant mouse embryonic fibroblasts （MEFs） were used as feeder layer cells. The mouse embryonic stem ceils （ES cells） were co-cultured with MEFs in media of DMEM supplement with leukemia inhibitory factor （LIF）. Results： The MEFs grew well and the ES cell colonies grew in small clusters and remain undifferentiated. Conclusions： The culture system of mouse ES ceils was established, which will provide the possibility for obtaining gene targeted ES cells.