中文摘要：

研究妊娠晚期兔羊膜上皮细胞（amniotic epithelial cells，AECs）在体外生长和增殖特性。取妊娠晚期兔（27-28E）AECs进行体外培养，光镜、扫描电镜下观察后，利用免疫组化单克隆抗体AE1／AE3、AE5检测培养的AECs中细胞角蛋白的表达，并采用流式细胞仪检测表皮生长因子（epidermal growth factor，EGF）和血清对AECs细胞周期的影响。结果表明妊娠晚期兔AECs在体外培养条件下生长良好、增殖旺盛；单克隆抗体AE1／AE3、AE5染色阳性；血清组、EGF组和联合应用组分别与对照组比较，各周期细胞比例发生变化，G0/G1期减少，S期、G2／M期增加，细胞增殖指数（PI）增加，P〈0．01，联合应用组分别与血清组、EGF组比较，P〈0．05。说明妊娠晚期兔AECs表达细胞角蛋白cK3，血清和EGF均能通过改变妊娠晚期兔AECs的细胞周期而促进AECs增殖，两者联合应用对促进AECs的增殖更为显著。

英文摘要：

Rabbit＇s amniotic epithelial cells （AECs） were obtained from rabbits at the late trimester of pregnancy （27-28E） and culturated in vitro. The AECs were observed under the light microscope and the scanning electron microscope. By using immunohistochemical staining, the AECs were examined to evaluate the expression of cytokeratin with monoclonal antibody AE1/AE3 and AE5. The influence of EGF and fetal bovine serum （FBS） on the cell cycle of AECs was determined by using flow cytometry. Rabbits＇ AECs at the late trimester of pregnancy grew well and proliferated actively in vitro. Immunohistochemical staining showed that the expression of AE1/AE3 and AE5 of the cultured AECs was positive. Compared with the control group, the percentage of cells at different phrase changed. Those at the G0/G1 phase decreased, while cells at the S phase and G2/M phase increased. The cell proliferation index （PI） increased, P〈0.01. The co-application of EGF and serum group was compared with the EGF group and FBS group respectively, P〈0.05. The AECs had cytokeratin CK3. Both of EGF and FBS could promote the proliferation of AECs and the influence of the co-application of EGF and serum on the proliferation was more significant.