中文摘要：

目的：探讨利用纤维蛋白胶作为支架构建组织工程人羊膜上皮细胞（HAECs）植片重建眼表的可行性。方法：取足月剖宫产胎盘羊膜，经胶原酶和胰蛋白酶消化后，获得HAECs。在体外构建的纤维蛋白胶片上培养HAECs，细胞融合成片后，利用气液界面复层化，采用倒置显微镜、组织切片、HE染色、细胞角蛋白免疫组织化学染色和扫描电子显微镜观察HAECs的生长情况。结果：HAECs在纤维蛋白胶表面生长良好，细胞呈圆形或多角形，长满后呈上皮细胞特有的铺路石样外观，扫描电镜观察细胞表面有丰富的微绒毛，细胞广谱角蛋白单克隆抗体染色阳性。细胞有复层生长趋势，植片较为透明。结论：以纤维蛋白胶为载体构建组织工程人HAECs植片，具有眼表重建的潜在运用价值。

英文摘要：

AIM： To investigate the feasibility of using human anmiotic epithelial cells （HAECs） sheets, which are cultured by using a biodegradable fibrin sealant to re-establish corneal surface layer. METHODS ： Human amniotic membrane （AM） harvested at the time of elective caesarean section was digested by collagenase and trypsin respectively to obtain HAECs. The HAECs were planted on MilliceU culture dishes coated with biodegradable fibrin glue, and cultured by using the air-lifting cultivation. The HAECs sheets were investigated morphologically by Hematoxylin-Eosin staining, scanning electron microscopy （SEM） and were identified by cytokeratin immunohistochemistry. RESULTS： HAECs sheets were transparent and successfully cultured by using a biodegradable fibrin sealant. Most of the cultured cells were round or polygon and typical slabstone - like appearance. Many microvilli were observed on cell surfaces by SEM. Cytokeratin staining was positive. HAECs had stratificated growth tendency and became corneal like stratificating epithelial cells. CONCLUSION： A commercially available fibrin sealant was an effective means of tissue engineering to create a carrier-free, transplantable HAECs sheets. The sheets were potential graft to re-establish corneal surface layer.