中文摘要：

目的合成1-苯基-3-甲基-4-苯甲酰吡唑啉酮-5-苯甲酰肼腙（HL）的Pd（Ⅱ）、Pt（II）配合物PDL：和PtL2，研究其对牛血清白蛋白（BSA）的影响。方法利用紫外吸收光谱和静态荧光光谱定性讨论了HL以及PDLz和PtLz对BSA构象的影响。结果HL以及PDL2和PtL2以静态猝灭的方式猝灭了BSA的内源荧光，并计算出了不同温度下这3种化合物与BSA的键合常数（Ka）以及热力学参数焓变（AH）及熵变（AS）。结论HL和PDL2与BSA的作用力主要是静电相互作用，而对于PtL2，除了静电相互作用外还包括疏水相互作用。

英文摘要：

Objective The complexes PdL2 and PtL2 were synthesized firstly by 1-phenyl-3-methyl-4-benzoyl pyrazolone-5-benzoyl hydrazine hydrazone（HL）and Pd（II） ,Pt（II）. HL was a bidentate and its N,S atoms bond met- al ions. Methods The effects of HL,PdL2 and PtL2 on the conformation of bovine serum albumin（BSA）were dis- cussed using UV absorption spectra and static fluorescence spectroscopy. Results The results showed that they quenched the endogenous fluorescence of BSA by static quenching style. The binding constants（Ka）and the thermo- dynamic parameters（the enthalpy change AH and the entropy change AS）between the three compounds and BSA at different temperature were calculated. Conclusion The thermodynamic parameters suggested that electrostatic in- teraction force were the main force between the two compounds（HL, PdL2 ）and BSA. However, hydrophobic inter- actions were also found between PtL2 and BSA besides electrostatic interaction force.