欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究低硒和/或低碘对子三代大鼠软骨细胞凋亡的影响。方法将48只断乳SD大鼠随机分为低硒组、低碘组、低硒低碘组和对照组4组,采用末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）技术和免疫组织化学方法检测各组子三代大鼠关节软骨细胞的凋亡率、Bcl-2和Bax蛋白表达及其分布情况。结果与对照组相比,低硒组、低碘组及低硒低碘组子三代大鼠关节软骨表层和中层软骨细胞凋亡增多,尤以中层增多更显著（P〈0.05）,低硒组、低碘组及低硒低碘组间细胞凋亡阳性率差异无统计学意义（P〉0.05）;低硒组、低碘组及低硒低碘组子三代大鼠关节软骨表层和中层Bcl-2和Bax表达阳性细胞数较对照组明显增多（P〈0.05）,低硒组、低碘组及低硒低碘组间Bcl-2和Bax表达阳性细胞数差异无统计学意义（P〉0.05）。结论低硒和/或低碘可能诱导大鼠关节软骨细胞异常凋亡。
英文摘要：
Objective To explore the effects of selenium and/or iodine deficiency on chondrocyte apoptosis in articular cartilage in rats. Methods Forty-eight Spraguee-Dawley rats were randomly divided into selenium deficiency group, iodine deficiency group, combined selenium and iodine deficiency group, and control group. Chondrocyte apoptosis was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling （TUNEL） method, and Bcl-2 and Bax in articular cartilage were stained by immunohistochemistry in F3 generation of rats. Results In articular cartilage , the positive rate of apoptotic chondrocytes stained by TUNEL in the upper and middle zones in selenium deficiency group, iodine deficiency group, and combined selenium and iodine deficiency group （ all P 〈 0. 05 ） were significantly higher than that in control group. The apoptotic chondrocytes were prominent in the middle zone. The positive percentage of chondrocytes apoptosis was not significantly different among these three groups （ P 〉 0. 05 ）. Compared with the control group, the expressions of both Bcl-2 and Bax were significantly higher in the upper and middle zone in the selenium deficiency group, iodine deficiency group, and combined selenium and iodine deficiency group （ all P 〈 0.05 ） ; however, the expressions of Bcl-2 and Bax were not significantly different among these three groups （P 〉 0. 05 ）. Conclusion Selenium and/or iodine deficiency may induce chondrocyte apoptosis.
同期刊论文项目
同项目期刊论文
Different challenge terms determine disease patterns of antigen-induced pulmonary inflammation in E3
Detection of serum proteomic changes and discovery of serum biomarkers for Kashin-Beck Disease using
Kashin-Beck disease and Sayiwak disease in China: Prevalence and a comparison of the clinical manife
Measuring health-related quality of life in Kashin-Beck disease using EQ-5D. Qual Life Res. 2011;20(
Heritability estimates and linkage analysis of 23 short tandem repeat loci on chromosomes 2, 11, and
Suppressive effects of black seed oil on ovalbumin induced acute lung remodeling in E3 rats. Swiss M
Main source of drinking water and familial aggregation of Kashin-Beck disease: a population based on
Oxidant Damage in Kashin-Beck disease and a rat Kashin-Beck disease model by employing T-2 toxin tre
Mitochondrial function is altered in articular chondrocytes of an endemic osteoarthritis, KashineBec
Genome-Wide Gene Expression Analysis Suggests an Important Role of Hypoxia in the Pathogenesis of En
Histopathology of Chondronecrosis development in knee articular cartilage in a rat model of Kashin-B
Correlation analysis between bone mineral density and serum element contents of postmenopausal women
The Relationship between Endothelial Nitric Oxide Synthase Gene Polymorphisms and Osteoporosis in Ch
Morphology and phenotype expression of types I, II, III, and X collagen and MMP-13 of chondrocytes c
Proteomic changes in articular cartilage of human endemic osteoarthritis in China. Proteomics. 2011.
A Modified Method using TRIzol((R)) Reagent and Liquid Nitrogen Produces High-Quality RNA from Rat P
Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoart
T-2 toxin induced apoptosis involving NO, P53, Bcl-xL, Bcl-2, Bax and Caspase-3 signaling pathway in
Analysis of correlation between blood biochemical indicators and bone mineral density of post-menopa
High Expression of Liver Histone Deacetylase 3 Deteriorates High-fat-diet-induced Metabolic Syndrome
The effect of short tandem repeat loci and low selenium levels on endemic osteoarthritis in China. J
A systematic comparison between collagen-induced arthritis and pristane-induced arthritis in Dark Ag
Nitric oxide both in bronchoalveolar lavage fluid and serum is assocaited with pathogenesis and seve
Downregulated HS6ST2 in osteoarthritis and Kashin–Beck disease inhibits cell viability and influence
期刊信息
位置：