中文摘要：

目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。方法：采用分子克隆方法构建GLUT4-EGFP的融合蛋白，在FLP—in的CHO细胞系中表达，潮霉素筛选后得到稳定的细胞系。结果：通过共聚焦显微镜的检测，证明了此稳定细胞系的阳性率达到了99％。定位研究表明大部分GLUT4以囊泡形式分布在CHO细胞胞浆内，但是质膜上也有少量的GLUT4。结论：建立了一个稳定表达GLUT4-EGFP的CHO细胞系，为进一步研究GLUT4的转运提供了一个很好的细胞模型。

英文摘要：

Objective： To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells. Methods： Recombinant plasmid pcDNA5/FRT-GLUT4-EGFP was constructed, co-transfected with pOG44 into FLP-in-CHO cell by lipofectamine, positive clones were screened by Hygromycin. Results： Confocal microscopy image showed that over 99% CHO cells expressed GLUT4-EGFP. Further distribution results showed that most of GLUT4 was stored within vesicles in cytosol in CHO cells, but a little GLUT4 was located on the plasma membrane. Conclusion： A stable CHO cell line expressing GLUT4-EGFP was established, which provided a good cell model for studying GLUT4 function and the mechanisms of GLUT4 translocation.