中文摘要：

目的以分离纯化的42株野生大豆内生真菌为材料,分析内生真菌菌丝及发酵液中次生代谢产物的积累,筛选高产次生代谢产物内生菌株。方法采用液体发酵摇瓶培养法及紫外分光光度法对不同内生真菌菌丝及发酵液中的总三萜、黄酮、多糖、多酚与总酚的量进行检测。结果 42株野生大豆内生真菌菌丝中,筛选获得高质量分数多糖菌株为Y2S9、WDL3和WDL2,质量分数最高可达85.51 mg/g;高质量分数黄酮菌株为Y1S9和Y6S8,质量分数分别为2.99和2.24 mg/g;高质量分数三萜菌株为Y6S8、Y2R1、Y6R2,质量分数分别为25.55、17.89、15.96 mg/g;而WDS7总酚量最高,为22.65 mg/g。内生真菌发酵液中,黄酮量较高菌株为Y1S8和SYS4,其质量浓度分别为3.11和4.81 mg/L;总三萜质量浓度较高的菌株为YSYL2、Y1S8、WDL3,质量浓度分别为17.46、35.00、42.98 mg/L。结论不同生境可能是导致次生代谢产物量差异的主要原因,本研究筛选出高产次生代谢产物野生大豆内生真菌菌株11株,为野生大豆内生真菌功能挖掘及新药筛选奠定了基础。

英文摘要：

Objective Using 42 endophytes separated and purified from wild soybean as materials, the accumulation of secondary metabolites in hypha and fermentation liquid of endophytic fungi was analyzed so as to screen the strains with high content of secondary production. Methods Liquid fermentation cultivation with shaking flask and ultraviolet spectrophotometer was utilized to detect the contents of total triterpenoids, flavonoids, polysaccharides, polyphenols, and total phenolic in different endophyte mycelia and their ferment liquids. Results For 42 endophytic fungi from wild soybean, strains Y2S9, WDL3, and WDL2 had high content of polysaccharide up to 85.51 mg/g, then Y1S9 and Y6S8 had higher content of the flavonoid with 2.99 and 2.24 mg/g, respectively; In addition Y6S8, Y2R1, and Y6R2 would produce more triterpenoid with 25.55, 17.89, and 16.39 mg/g; Meanwhile WDS7 had the highest content of total phenol with 22.65 mg/g. For the fermentation liquid of endophytic fungi, strains Y1S8 and SYS4 had higher contents of flavonoid with 3.111 and 4.809 mg/L; But strains YSYL2, Y1S8, and WDL3 had the higher levels of total triterpenoids up to 17.46, 35.00, and 42.98 mg/L, respectively. Conclusion Different habitats might be the main factor causing difference in the contents of secondary production. Eleven strains with high yield secondary metabolites selected from wild soybean would lay the foundation for mining endophytic fungi function and screening new drugs.