从SBR活性污泥中筛选、分离出1株有聚-β羟基丁酸酯(PHB)积累能力的聚磷细菌(将其命名为“LB4”),通过形态、生理生化及16SrDNA方法分析,鉴定该聚磷细菌为鲍曼不动杆菌似cinetobacter baumannii)。采用厌氧、好氧交替培养方法,研究影响其积累聚-β羟基丁酸酯(PHB)的因素。结果表明:在厌氧初始阶段,当pH为8.0,COD为2000mg/L,培养温度为35℃时,通过批式补料方式短期限制磷元素对其进行培养,最有利于该菌株积累PHB,PHB的最高积累量可达菌体干重的32.6%。
LB4, a phosphate-accumulating strain with the ability of poly-β-hydroxybutymte(PHB) granules accumulation, was isolated from activated sludge of SBR in treatment equipment of wastewater. Through the analysis from morphological, biochemical characteristics, and identification using 16S rDNA sequence approach, LB4 was proved to be a subspecies ofAcinetobacter baumannii. The influence factors for PHB accumulation were investigated by alternative cultivation of anaerobic-aerobic cycle. The results showed that the favourite conditions for the strain to accumulate PHB at the beginning of anaerobic phase was at temperature 35 ℃, pH 8.0, COD 2 000 mg/L, and with short-term phosphorus inhibition, the maximum accumulation of PHB could reach to that of 32.6% dry weight of the strain.