中文摘要：

目的：探讨钒配合物LMc对拓扑异构酶Ⅰ、Ⅱ（Topo-Ⅰ、Topo-Ⅱ）的影响及其抗肿瘤活性。方法：采用DNA松弛实验观察LMC对Topo-Ⅰ、活性的影响并探讨其相关分子作用机制；采用MTT法、流式细胞术在细胞水平观察了IMC的抗肿瘤作用。结果：LMC可明显抑制Topo-Ⅰ活性，对Topo-Ⅱ无明显抑制作用，对多种肿瘤细胞株A549、Hela、BEL-7402具有明显抑制生长的作用，且可将细胞阻断在G2／M期，而对正常细胞株L-02生长无明显影响。结论：钒配合物LMC具有抑制Topo-Ⅰ活性而发挥抗肿瘤的作用。

英文摘要：

In this paper, we studied the effects of vanadium compound LMC on DNA topoisomerase and its antitumor activity. DNA relaxation assay was used to detect the activity of LMC on topoisomerase Ⅰ （Topo - Ⅰ ） and topoisomerase Ⅱ （Wopo - Ⅱ ） and its correlated molecular mechanism of action; MTr assay and Flow cytometry were used to detect LMC＇ s antitumor activity from cytology angle. LMC can inhibit Topo- Ⅰ activity obviously, but not, Topo - Ⅱ LMC can also inhibit A549,Hela,BEL- 7402 cell lines growth evidently, and LMC can arrest the ceils at G2/M stage. However, it makes no difference to normal cell llne L- 02, from which we conclude that Vanadium compound LMC has obvious anticancer activity through inhibiting Topo- Ⅰ activity.