中文摘要：

本研究以不同溶剂和方法对核桃青皮进行了抗植物病毒物质的提取和活性测定，并且通过单因素试验和正交试验，以核桃青皮中抗病毒活性物质的抑制率为主要考察指标，对影响提取效果的各因素进行了研究。结果表明：提取物的抗病毒活性因提取溶剂和提取方法的不同而异。以体积分数95％乙醇为提取溶剂，冷浸提取物在质量浓度10mg／mL时，对烟草花叶病毒（Tobacco mosaicvirus，TMV）的抑制率最高，为80．0%。采用冷浸提取时，各因素对核桃青皮抗病毒活性物质提取效果的影响程度从高到低依次为乙醇浓度〉液固比〉提取次数〉提取时间，确定了优化的提取条件为：95%乙醇冷提4次，每次26h，液固比为5：1。

英文摘要：

To optimize the conditions for extracting antiviral substances from walnut （Juglans regia L） peel, different solvents and methods were applied. The extraction process was studied by single factor and orthogonal experiment ：guided by the inhibition rate of antiviral substances. The results showed that activities of antiviral substances varied from different solvents and methods. The extract from 95 % ethanol with a concentration of 10 mg/mL, exhibited the highest inhibition rate on tobacco mosaic virus （TMV） i.e. , 80. 0 %. The successive order of factors affecting the extracting was ethanol concentration, liquid-solid ratio, extracting times, extracting time. The optima conditions of soaking were determined to be 95 % alcohol 26 h,4 times,and 5 ： 1 liquid-solid ratio.